ON-LINE DETERMINATION OF CLOTTING TIME IN GOAT’S MILK BY BACKSCATTER OF NEAR INFRARED RADIATION
Abstract
An on-line fiber optic sensor has been used to monitor goat’s milk coagulation. The sensor determined the diffuse reflectance increase (880 nm) due to the physical and chemical modifications of casein micelles, which caused the gel formation. The results obtained showed a high correlation (R2 = 0.99) between the diffusereflectance variable, Tmax, and the Berridge clotting time, the main reference parameter in the study of enzymatic activity of coagulating enzymes. It was observed that Tmax is an 89% of Berridge clotting time, which was related to a bigger sensitivity of Tmax to aggregation. It was estimated that the percentage of k casein hydrolysis at Tmax is 78%. The results demonstrated that Tmax not only responded to enzyme concentration changes as predicted by the Foltmann equation but also changed consistently with other coagulation factors. For this reason, and for its high sensitivity to aggregation reaction, Tmax was proposed as an accurate alternative to Berridge method, which justify the usefulness of the backscatter sensor as a process control tool.Downloads
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