CHOLINESTERASE STABILITY OF WHOLE BLOOD SAMPLES FROM DIVERSE ANIMAL SPECIES UNDER DIFFERENT STORAGE CONDITIONS
Abstract
In this work, study of cholinesterase stability in whole blood samples from different animal species and determination of storage conditions that allow maximum enzyme conservation is pretended. Canine, equine and ovine whole blood samples were stored at 25º, 4º and –20º C with and without previous dilution in distilled water at 1:50 dilution degree. Cholinesterase activity was monitored at the same day of samples collection and 1 day, 3 days, 1 week, 2 weeks, 1 month, 3 months and 6 months of storage, using the adapted Ellman method for an automated spectrophotometer (Coulter Profile Analyzer). Simultaneously, canine whole blood samples were frozen and thawed once a day during five consecutive days, in order to evaluate the effect on cholinesterase activity. Maximum cholinesterase stability was observed when whole blood was stored at –20º C in canine and ovine samples, and at 4º C in equine samples. Rapid lose of cholinesterase activity was observed at 25º and 4º C in diluted blood; whereas dilution yielded higher stability in ovine, lesser in equine, and similar to non-diluited samples in canine blood. Successive whole blood freezing and thawing did not significantly affect enzyme activity.Downloads
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