Deterioration and health quality indicators in refrigerated fillets of sea bream treated with ozonated water and glycine salt

Authors

  • María Jesús Periago Castón Universidad de Murcia
  • Marina Santaella Pascual Universidad de Murcia
  • Carmen Martínez-Graciá Universidad de Murcia
  • I. Navarro-González Universidad de Murcia
  • C. Puche Universidad de Murcia
Keywords: sea bream, tvnb, tma-n, spoilage bacteria, microbiological quality

Abstract

In this study we have studied the effect of washing with ozonized water and the treatment with a commercial glycine salt at 5 ppm for 2 min., on the quality parameters of sea bream fillets (Sparus aurata). After treatment, the fillets were introduced in a plastic bag and stored in ice and in refrigeration, carrying out the analysis at time cero and at 6, 10 and 17 days of storage. Total volatile Basic Nitrogen (TVBN) and Trimethylamine nitrogen (TMA-N) were analyzed as chemical indicators. The microbiological quality was determined through the isolation and quantification of the hygiene microorganisms (total aerobic mesophiles), Enterobacteriaceae and coliforms) and specific microorganisms of the fish spoilage of (Pseudomonas spp., Brochotrix thermosphacta y Shewanella putrefaciens). During the refrigerated storage a significant increase of the different microorganisms and of chemical indicators were observed in the sea bass fillets, reaching unacceptable levels after the 10 days of storage. In general, the utilization of the commercial glycine salt at 5 ppm do not exhibit an effect on the reduction of the specific microorganisms of sea bass fillet spoilage in comparison with the samples washing only with ozonized water.

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References

ALASALVAR, C., TAYLOR, K. D. A., OKSUZ, A., GARTWAITE, T., ALEXIS, M. N., GRIGOAKIS, K. 2001. Freshness assessment of cultured sea bream (Sparus aurata) by chemical, physical and sensory methods. Food Chem. 7: 33-40.

ATREA, I., PAPAVERGOU, A., AMVROSIADIS, I., SAVVAIDIS, I. N. 2009. Combined effect of vacuum-packaging and oregano essential oil on the shelf-life of Mediterranean octopus (Octopus vulgaris) from the Aegean Sea stored at 4 °C. Food Microbiol. 26:166-172.

CAKLI, S., KILINC, B., CADUN A., DINCER T., TOLASA S. 2006. Effects of using slurry ice on the microbiological, chemical and sensory assessments of aquacultured sea bass (Dicentrarchus labrax) stored at 4°C. Eur. Food Res. Techn. 222:130-138.

CAKLI, S., KILINC, B., CADUN, A., DINCER, T., TOLASA, S. 2007. Quality diferences of whole ungutted sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) while stored in ice. Food Control 18:391–397.

CARUSO, G., MAIMONE, G., MANCUSO, M., MODICA, A., GENOVESE, L. 2004. Microbiological controls across the productive cycle of Dicentrarchus labrax L. and Sparus aurata L.: a study from the environment to the final product. Aquacul. Res. 35:184-193.

CASTRO, P., PENEDO, J. C., CABALLERO CANSINO, M. J., SANJUÁN, E., MILLÁN, R. 2006. Total volatile base nitrogen and its use to assess freshness in European sea bass stored in ice. Food Control 17:245-248.

CHOULIARA, I., SAVVAIDIS, I. N., RIGANAKOS, K., KONTOMINAS, M. G. 2005. Shelf-life extension of vacuum-packaged sea bream (Sparus aurata) fillets by combined γ -irradiation and refrigeration: microbiological, chemical and sensory changes. J. Sci. Food Agric. 85:779-784.

CHYTIRI, S., CHOULIARA, I., SAVVAIDIS, I. N., KONTOMINAS, M. G. 2004. Microbiological, chemical and sensory assessment of iced whole and filleted aquacultured rainbow trout. Food Microbiol. 21:157-165.

DALGAARD, P., MADSEN, H.L., SAMIEIAN, N., EMBORG, J. 2005. Biogenic amine formation and microbial spoilage in chilled garfish (Belone belone belone) – effect of modified atmosphere packaging and previous frozen storage. J. Appl. Microbiol. 101(1):80-95.

EMBORG, J., LAURSEN, B. G., DALGAARD, P. 2004. Significant histamine formation in tuna (Thunnus albacares) at 2°C -effect of vacuum- and modified atmosphere-packaging on psychrotolerant bacteria. Int. J. Food Microbiol. 101:263-279.

ERKAN, N., G. RETENER, U., ALPAS, H. 2010. Effects of high-pressure treatment on physicochemical characteristics of fresh sea bass (Dicentrarchus labrax). J. Consumer Protec. Food Safety 5:83-89.

ERKAN, N., RETENER, G. U. 2010. The effect of high hydrostatic pressure on the microbiological, chemical and sensory quality of fresh gilthead sea bream (Sparus aurata). Eur. Food Res. Technol. 230:533-542.

FERNÁNDEZ-SEGOVIA, I., ESCRICHE, I., FUENTES, A., SERRA, J.A. 2006. Microbial and sensory changes during refrigerated storage of desalted cod (Gadus morhua) preserved by combined methods. Int. Food Microbiol. 116:64-72.

FRANGOS, L., PYRGOTOU, N., GIATRAKOU, V., NTZIMANI, A., SAVVAIDIS, I.N. 2010. Combined effects of salting, oregano oil and vacuum-packaging on the shelf-life of refrigerated trout fillets. Food Microbiol. 27:115-121.

FUENTES, A., FERNÁNDEZ-SEGOVIA, I., BARAT, J. M., SERRA, J. A. 2011. Influence of sodium replacement and packaging on quality and shelf life of smoked sea bass (Dicentrarchus labrax L.). Food Sci. Technol. 44:917-923.

GOULAS, A. E., KONTOMINAS, M. G. 2007. Combined effect of light salting, modified atmosphere packaging and oregano essential oil on the shelf-life of sea bream (Sparus aurata): Biochemical and sensory attributes. Food Chem. 100:287-296.

GRAM, L., MELCHIORSEN, J. 1996. Interaction between fish spoilage baceteria Pseudomonas sp. and Shewanella putrefaciens in fish extracts and on fish tissue. J Appl. Bacteriol. 80:589-595.

ICMSF (International Commission on Microbiological Specifications for Foods), 1998. Microorganisms in Foods. En: Microbial Ecology of Food Commodities-Fish and Fish Products, vol. 6, pp. 130-189.

ICMSF (International Commission on Microbiological Specifications for Foods), 1986. Sampling plans for fish and shellfish. En: ICMSF, Microorganisms in Food Sampling for Microbiological Analysis: Principles and Scientific Applications, vol. 2, pp. 181-196.

LÓPEZ-CABALLERO, M. E., HUIDROBO, A., PASTOR, A., TEJADA, M. 2002. Microflora of gilthead seabream (Sparus aurata) stored in ice. Effect of washing. Eur. Food Res. Technol. 215:396-400.

LYHS, U., LAHTINEN, J., FREDRIKSSON-AHOMAA, M., HYYTI-TREES, E., ELFING, K., KORKEALA, H. 2001. Microbiological quality and shelf-life of vacuum-packaged ‘gravad’ rainbow trout stored at 3 and 8°C. Int. J. Food Microbiol. 70:221-230.

MASNIYOM, P., BENJAKUL, S., VISESSANGUAN, W. 2002. Shelf-life extension of refrigerated seabass slices under modified atmosphere packaging. J. Sci. Food Agric. 82:873-880.

MASNIYOMA, P., BENJAKULA, S., VISESSANGUAN, W. 2004. Combination effect of phosphate and modified atmosphere on quality and shelf-life extension of refrigerated seabass slices. Lebensm. Wissen. Technol. 38:745-756.

MEJLHOLM, O., BØKNÆS, N., DALGAARD, P. 2004. Shelf life and safety aspects of chilled cooked and peeled shrimps (Pandalus borealis) in modified atmosphere packaging. J.Appl. Microbiol. 99:66-76.

MENDES, R., GONZALVES, A. 2007. Effect of soluble CO2 stabilization and vacuum packaging in the shelf life of farmed sea bream and sea bass fillets. Int. J. Food Sci. Technol. 43:1678-1687.

MOHAN, C. O., RAVISHANKAR, C. N., SRINIVASA GOPAL, T. K., LALITHA, K. V., ASOK KUMAR, K. 2010. Effect of reduced oxygen atmosphere and sodium acetate treatment on the microbial quality changes of seer fish (Scomberomorus commerson). Food Microbiol. 27:526-534.

OLOFSSON, T. C., AHRNE, S., MOLIN, G. The bacterial flora of vacuum-packed cold-smoked salmon stored at 7°C, identified by direct 16S rRNA gene analysis and pure culture technique. J. Appl. Microbiol. 103:109-119.

PALEOLOGOS, E. K., SAVVAIDIS, I. N., KONTAMINAS, M. G. 2004. Biogenic amines formation and its relation to microbiological and sensory attributes in ice-stored whole gutted and filleted Mediterranean Sea bass (Dicentrarchus labrax). Food Microbiol. 21:549-557.

PAPADOPOULOS, V., CHOULIARA, I., BADEKA, A., SAVVAIDIS, I. N., KONTOMINAS, M. G. 2003. Effect of gutting on microbiological, chemical and sensory properties of aquacutured sea bass (Dicentrarchus labrax) stored in ice. Food Microbiol. 20:411-420.

TALIADOUROU, D., PAPADOPOULOS, V., DOMVRIDOU, E., SAVVAIDIS, I. N., KONTAMINAS, M. G. 2003. Microbiological, chemical and sensory changes of whole and filleted Mediterranean aquacultured sea bass (Dicentrarchus labrax) sotred in ice. J. Sci. Food Agric. 83:1373-1379.

VAIKOUSI, H., BILIADERIS, C. G., KOUTSOUMANIS, K. P. 2009. Applicability of a microbial Time Temperature Indicator (TTI) for monitoring spoilage of modified atmosphere packed minced meat. Int. J. Food Microbiol. 133:272-278.

ZOGUL, F. O., KULEY, E., YESIM, O. 2007. Sensory, chemical and microbiological quality parameters in sea bream (Sparus aurata) stored in ice or wrapped in cling film or in aluminum foil at 2 ± 1°C. Int. J. Food Sci. Technol. 42:903-909.

Published
20-03-2019
How to Cite
Periago Castón, M. J., Santaella Pascual, M., Martínez-Graciá, C., Navarro-González, I., & Puche, C. (2019). Deterioration and health quality indicators in refrigerated fillets of sea bream treated with ozonated water and glycine salt. Anales de Veterinaria de Murcia, 32, 45–58. Retrieved from https://revistas.um.es/analesvet/article/view/369171
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