EFFECT OF SPERM PREPARATION PROTOCOL ON THE BOVINE ACROSOME REACTION PATTERN
Abstract
The acrosome reaction of bovine frozen-thawed semen samples, under different preparation processes, was analysed by the lectin binding staining. The addition of heparin and caffeine to the medium significantly increased the number of sperm with acrosome reaction. When sperm was selected by the use of a Percoll gradient column, the process of acrosome reaction was accelerated. These data confirm that the sperm preparation for the in vitro fertilisation has an effect on the capacitation and acrosome reaction pattern, and it could be determinant for the fertilisation process.Downloads
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