Effect of preventive and simultaneous inoculations of Bacillus amyloliquefaciens (Fukumoto) strains on conidial germination of Botrytis cinerea Pers.:Fr.
Abstract
Botrytis cinerea is a necrotrophic fungal plant pathogen distributed worldwide. In the current study, the effect of nine bacterial isolates, on germination of B. cinerea conidia were studied. The nine isolates were identified as Bacillus amyloliquefaciens. The efficacy of isolates was tested, at different concentrations, in preventive and simultaneous inoculations with B. cinerea conidia. Results showed that five Bacillus isolates were more effective when co-inoculated simultaneously with the pathogen. Four isolates showed more efficacies when inoculated previously four hours before the pathogen. The isolate denominated B24 was the only that showed an important percent inhibition (84.04 %) at the lower concentration tested (3x103 CFU/ml) and the isolate RA9 was the less effective strain.
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