Oral lipid loading test in Wistar rats as a reliable method for the study of the triacylglycerol malabsorption
Abstract
Most of the lipid digestion is carried out by pancreatic lipase, secreted in the upper part of the intestinal lumen by pancreatic juice secretions. In this paper, we describe a new single stressless and fast method to evaluate plasma triacylglycerol levels on Wistar rats under anaesthesia and with minimal personal training. After the administration of different diet treatments, plasma triacylglycerol (TAG) levels were analyzed at 30, 60, 120, 180 minutes to characterize the lipidic gastrointestinal absorption. Orlistat, a known antiobesity drug, was used as positive control. The results shown that the method was able to differentiate the treatments administered utilizing up to five animals at each time step. In addition, with this method was shown that Orlistat treatment prevents TAG systemic uptake at 30 and 60 minutes compared to a control diet without it. On the light of these results we conclude that the method described in this study could be employed to evaluate short-term lipid digestion.Downloads
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