Efecto de inoculaciones preventivas y simultáneas de cepas de Bacillus amyloliquefaciens (Fukumoto) sobre la germinacion de conidios de Botrytis cinerea Pers.:Fr.
Resumen
Botrytis cinerea es un patógeno fúngico necrotrófico de varias plantas distribuido en todo el mundo. En el presente estudio se analizó el efecto de nueve aislados bacterianos sobre la germinación de conidios de B. cinerea. Los aislados se identificaron como Bacillus amyloliquefaciens. Para evaluar la eficacia de los aislados, se realizaron confrontaciones duales tanto preventivas como simultáneas con los conidios de B. cinerea. Los resultados mostraron que cinco aislados fueron más eficaces cuando se co-inocularon simultáneamente con el patógeno. Cuatro aislados fueron más eficaces cuando se inocularon previamente cuatro horas antes el patógeno. La cepa denominada B24, mostró una inhibición de (84.04%) en concentraciones muy bajas (3x103 UFC/ml) y la cepa RA9 fue la cepa menos eficaz.
Descargas
Citas
Agrios GN. 2005. Plant Pathology. 5th édition, p : 922.
Arguelles-Arias A, Ongena M, Halimi B, Lara Y, Brans A, Joris B. & Fickers P. 2009. Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens. Microbial Cell Factories 26: 8-63.
Blakeman JP & Brodie I. 1977. Competition for nutrients between epiphytic microorganisms and germination of spores of plant pathogens on beet root leaves. Physiological Plant Pathology 10: 29-42.
Botton B, Breton A, Fèvre M, Gauthier S, Guy P, Larpent JP, Reymond P, Sanglier JJ, Vayssier Y & Veau P. 1990. Moisissures utiles et nuisibles. Importance industrielle. 2e éd. revue et complétée. Paris, Milan, Barcelone: Masson, p. 512.
Braun PG & Sutton JC. 1987. Inoculum sources of Botrytis cinerea in fruit rot of strawberries in Ontario. Canadian Journal of Plant Pathology 9: 1-5.
Chowdhury SP, Hartmann A, Gao XW & Borriss R. 2015. Biocontrol mechanism by root-associated Bacillusamyloliquefaciens FZB42–areview. Frontiers in Microbiology 6: 780.
Dik AJ & Elad Y. 1999. Comparison of antagonists of Botrytis cinerea in greenhouse-grown cucumber and tomato under different climatic conditions. European Journal of Plant Pathology 105: 123-137.
Elad Y & Chet I. 1987. Possible role of competition for nutrients in biocontrol of Pythium damping-off by bacteria. Phytopathology 77: 190-195.
Elad Y & Stewart A. 2004. Microbial control of Botrytis spp.. In: Botrytis: Biology, Pathology and Control. (Y. Elad, B. Williamson, P. Tudzynski, N. Delen, eds.), Kluwer Academic Publishers, Dordrecht, Netherlands, 223-241.
Elad Y, Köhl J & Fokkema NJ. 1994. Control of infection and sporulation of Botrytis cinerea on bean and tomato by saprophytic yeasts. Phytopathology 84: 1193-1199.
Elad Y. 1996. Mechanisms involved in the biological control of Botrytis cinerea incited diseases. European Journal of Plant Pathology 102: 719-732.
Etcheverry M, Scandolara A, Nesci A, Vilas Boas Ribeiro MS, Pereira P & Battilani P. 2009. Biological interactions to select biocontrol agents against toxigenic strains of Aspergillus flavus and Fusarium verticillioides from maize. Mycopathologia 167(5): 287-295.
Ezziyyani M, Requena ME, Egea-Gilabert C & Candela ME. 2007. Biological Control of Phytophthora Root Rot of Pepper Using Trichoderma harzianum and Streptomyces rochei in Combination. Journal of Phytopathology 155 : 342-349.
Hahn M. 2014. The rising threat of fungicide resistance in plant pathogenic fungi: Botrytis as a case study. Journal of Chemical Biology 7: 133-141.
Hamdache A, Ezziyyani M, Badoc A & Lamarti A. 2012. Effect of pH, Temperature and Water Activity on the inhibition of Botrytis cinerea by Bacillus amyloliquefaciens isolates. African Journal of Biotechnology 11 (9) : 2210-2217.
Hamdache A, Lamarti A, Aleu J & Collado IG. 2011. Non-peptide Metabolites from the Genus Bacillus. Journal of Natural Product 74 (4) : 893-899.
Hamdache A, R. Azarken, Lamarti A, Aleu J & Collado IG. 2013. Comparative genome analysis of Bacillus spp. and its relationship with bioactive nonribosomal peptide production. Phytochemistry12: 685–716.
Holz G, Coertze S & Williamson B. 2004. The ecology of Botrytis on plant surfaces, in Elad Y, Williamson B, Tudzynski P, Delen N, editors. (ed), Botrytis: biology, pathology and control. Kluwer Academic Publishers, Dordrecht, Netherlands, pp. 9-27.
Ji SH, Paul NC, Deng JX, Kim YS, Yun B-S & Yu SH. 2013. Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases. Mycobiology 41(4): 234-242.
Leelasuphakul W, Sivanunsakul P & Phongpaichit S. 2006. Purification, characterization and synergistic activity of β-1,3-glucanase and antibiotic extract from an antagonistic Bacillus subtilis NSRS 89-24 against rice blast and sheath blight pathogens. Enzyme and Microbial Technology 38: 990-997.
Leroux P. 2004. Chemical control of Botrytis and its resistance to chemical fungicides. In: Botrytis: Biology, Pathology and Control. (Y. Elad, B. Williamson, P. Tudzynski, N. Delen, ed.), Kluwer Academic Publishers, Dordrecht, Netherlands, pp. 195-222.
M’piga P, Bélanger RR, Paulitz TC & Benhamou N. 1997. Increased resistance to Fusarium oxysporum f. sp. radicis-lycopersici in tomato plants treated with the endophytic bacterium Pseudomonas fluorescens strain 63-28. Physiological and Molecular Plant Pathology 50: 301-320.
Mari M, Guizzardi M & Pratella GC. 1996. Biological control of gray mold in pears by antagonistic bacteria. Biological Control 7: 30-37.
Mohamed S & Caunter IG. 1995. Isolation and characterization of Pseudomonas fluorescens strain suppressive to Bipolaris maydis. Journal of Phytopathology 143: 111-114.
Morris CE & Rouse DI. 1985. Role of nutrients in regulating epiphytic bacterial populations. Biological Control on the Phylloplane. C. E. Windels and E. Lindow, eds. The American Phytopathological Society, St. Paul, MN, pp. 63-82.
Rabosto X, Carrau M, Paz A, Boido E, Dellacassa E, Carrau FM. 2006. Grapes and vineyard soils as sources of microorganisms for biological control of Botrytis cinerea. American Journal Enology and Viticulture 57: 332-338.
Roberts RG. 1990. Postharvest biological control of gray mold of apple by Cryptococcus laurentii. Phytopathology 80: 526-530.
Romanazzi G & Feliziani E. 2014. Botrytis cinerea. In: Postharvest Decay: Control Strategies (S. Bautista-Baños, ed.), Elsevier, London, 131-146.
Saligkarias ID, Gravanis FT and Eptona HAS. 2002. Biological control of Botrytis cinerea on tomato plants by the use of epiphytic yeasts Candida guilliermondii strains 101 and US 7 and Candida oleophila strain I-182: II. a study on mode of action. Biological Control 25: 151-161.
Samson RA, Hoekstra ES, Van Oorschot CAN. 1984. Introduction to food-borne fungi. 2nd edition. Centraalbureau Voor Schimmelcultures, BAARN. Institute of the Royal Netherlands, Academy Arts Sci. pp. 248.
Stein T. 2005. Bacillus subtilis antibiotics: structures, syntheses and specific functions. Molecular microbiology 56: 845-857.
Sturz AV, Christie BR & Matheson BG. 1998. Association of bacterial endophyte populations from red clover and potato crops with potential for beneficial allelopathy. Canadian Journal of Microbiology 44: 162-167.
Sutton JC. 1995. Evaluation of micro-organisms for biocontrol: Botrytis cinerea and strawberry, a case study. In: Advances in Plant Pathology, eds. J. H. Andrews, and I. C. Tommerup, Academic Press, San Diego, USA.
Toure Y, Ongena M, Jacques P, Guiro A & Thonart P. 2004. Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple. Journal of Applied Microbiology 96: 1151-1160.
Walker AS, Micoud A, Rémuson F, Grosman J, Gredt M & Leroux P. 2013. French vineyards provide information that opens ways for effective resistance management of Botrytis cinerea (grey mould). Pest Management Science 69: 667–678.
Walker R, Innes CMJ & Allan EJ. 2001. The potential biocontrol agent Pseudomonas antimicrobica inhibits germination of conidia and outgrowth of Botrytis cinerea. Letters in Applied Microbiology 32: 346-348.
Wisniewski ME & Wilson CL. 1992. Biological control of postharvest diseases of fruits and vegetables: recent advances. HortScience 27: 94-98.
Wu L, Wu H, Chen L, Yu X, Borriss R & Gao X. 2015. Difficidin and bacilysin from Bacillus amyloliquefaciens FZB42 have antibacterial activity against Xanthomonas oryzae rice pathogens. Scientific Reports 5: 12975.
Zhang Z, Jiang W, Jian Q, Song W, Zheng Z, Wang D & Liu X. 2015. Residues and dissipation kinetics of triazole fungicides difenoconazole and propiconazole in wheat and soil in Chinese fields. Food Chemistry. 168: 396-403.
Zouari I, Jlaiel L, Tounsi S & Trigui M. 2016. Biocontrol activity of the endophytic Bacillus amyloliquefaciensstrain CEIZ-11 against Pythium aphanidermatum and purification of its bioactive compounds. Biological Control 100: 54-62.
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
Las obras que se publican en esta revista están sujetas a los siguientes términos:
1. El Servicio de Publicaciones de la Universidad de Murcia (la editorial) conserva los derechos patrimoniales (copyright) de las obras publicadas, y favorece y permite la reutilización de las mismas bajo la licencia de uso indicada en el punto 2.
2. Las obras se publican en la edición electrónica de la revista bajo una licencia Creative Commons Reconocimiento-NoComercial-SinObraDerivada 3.0 España (texto legal). Se pueden copiar, usar, difundir, transmitir y exponer públicamente, siempre que: i) se cite la autoría y la fuente original de su publicación (revista, editorial y URL de la obra); ii) no se usen para fines comerciales; iii) se mencione la existencia y especificaciones de esta licencia de uso.
3. Condiciones de auto-archivo. Se permite y se anima a los autores a difundir electrónicamente las versiones pre-print (versión antes de ser evaluada) y/o post-print (versión evaluada y aceptada para su publicación) de sus obras antes de su publicación, ya que favorece su circulación y difusión más temprana y con ello un posible aumento en su citación y alcance entre la comunidad académica. Color RoMEO: verde.
Biological Abstracts, BIOSIS Previews, Zoological Record
