Effect of ultrasound on subserosal and intramural fibroids in vitro: A quasi-experimental study of physical therapy
Abstract
The aim of this study was to determine the effect of ultrasound (US) on extra subserosal and intramural uterine fibroids in vitro. A quasi-experimental, randomized, pre-post intervention study of physical therapy was conducted. Thirty women, who underwent myomectomy for subserosal or intramural fibroids with any leiomyomas' volumes in the operating room of Bab El-Sharia Hospital, participated in the study. Thirty uterine fibroids whose were collected from participants were randomly divided into three groups (I, II and III), and exposed to ultrasound therapy for 15, 30, and 45 minutes respectively. Leiomyomas volumes were measured using the fluid displacement method before and after the application of US. The samples were kept in saline solution immediately after their excision from operating room to be transferred to physical therapy department in same hospital to apply ultrasound therapy. Results of all study groups (I, II, and III) showed a statistically significant decrease in the volume of subserosal and intramural leiomyoma post US application (p>0.05). However, there was no statistically significant difference in the size of the subserosal and intramural leiomyoma between study groups (p<0.05). In conclusion, the ultrasound therapy is effective in shrinking the volume of subserosal and intramural leiomyomas without effect of long duration ultrasound therapy.
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References
Baker, K. G., Robertson, V. J., & Duck, F. A. (2001). A review of therapeutic ultrasound: biophysical effects. Physical therapy, 81(7), 1351-1358.
Beauchamp, R. D., Evers, B. M., Mattox, K. L., & Townsend, C. M. (2012). Sabiston Textbook of Surgery. E-Book.
Camanni, M., Bonino, L., Delpiano, E. M., Ferrero, B., Migliaretti, G., & Deltetto, F. (2010). Hysteroscopic management of large symptomatic submucous uterine myomas. Journal of Minimally Invasive Gynecology, 17(1), 59-65. https://doi.org/10.1016/j.jmig.2009.10.013
Dyson, M., Pond, J. B., Woodward, B., & Broadbent, J. (1974). The production of blood cell stasis and endothelial damage in the blood vessels of chick embryos treated with ultrasound in a stationary wave field. Ultrasound in medicine & biology, 1(2), 133-148. https://doi.org/10.1016/0301-5629(74)90003-9
El-Touny, E. (2015). Effect of ultrasound therapy on subserosal and intramural fibroids in premenopausal women. Lambert Academic Publishing.
Garg, K., Tickoo, S. K., Soslow, R. A., & Reuter, V. E. (2011). Morphologic features of uterine leiomyomas associated with hereditary leiomyomatosis and renal cell carcinoma syndrome: a case report. The American journal of surgical pathology, 35(8), 1235-1237. https://doi.org/10.1097/PAS.0b013e318223ca01
Giuliani, E., Sanie, S., & Marsh, E. E. (2020). Epidemiology and management of uterine fibroids. International Journal of Gynecology & Obstetrics, 149(1), 3-9. https://doi.org/10.1002/ijgo.13102
Gupta, S., & Manyonda, I. T. (2009). Acute complications of fibroids. Best Practice & Research. Clinical Obstetrics & Gynaecology, 23(5), 609-617. https://doi.org/10.1016/j.bpobgyn.2009.01.012
Hughes, S. W. (2005). Archimedes revisited: a faster, better, cheaper method of accurately measuring the volume of small objects. Physics Education, 40(5), 468.
Hynynen, K., McDannold, N., Sheikov, N. A., Jolesz, F. A., & Vykhodtseva, N. (2005). Local and reversible blood–brain barrier disruption by noninvasive focused ultrasound at frequencies suitable for trans-skull sonications. Neuroimage, 24(1), 12-20. https://doi.org/10.1016/j.neuroimage.2004.06.046
Jacoby, V. L., Autry, M., Jacobson, G., Domush, R., Nakagawa, M. S., & Jacoby, A. (2009). Nationwide use of laparoscopic hysterectomy compared with abdominal and vaginal approaches. Obstetrics and Gynecology, 114(5), 1041. https://doi.org/10.1097/AOG.0b013e3181b9d222
Jolesz, F. A., & McDannold, N. J. (2014). MRI-guided focused ultrasound. Intraoperative Imaging and Image-Guided Therapy, 60, 403-412.
Lethaby, A., Penninx, J., Hickey, M., Garry, R., & Marjoribanks, J. (2013). Endometrial resection and ablation techniques for heavy menstrual bleeding. Cochrane Database of Systematic Reviews, 8, 1-247.
Leung, J. H., Yu, S. C., Cheung, E. C., Wong, A. S., Tong, M. M., Ho, S. S., ... & Ahuja, A. T. (2014). Safety and Efficacy of Sonographically Guided High‐Intensity Focused Ultrasound for Symptomatic Uterine Fibroids: Preliminary Study of a Modified Protocol. Journal of Ultrasound in Medicine, 33(10), 1811-1818. https://doi.org/10.7863/ultra.33.10.1811
Mansour, S. E. D., Mohamed, M., Hegah, M., Okeel, F., Youssef, A., & Fouad, N. A. F. (2009). Effect of ultrasound therapy on alleviating pain and adhesions in endometriosis. The Egyptian Journal of Fertility & Sterility, 13(1), 13-20.
McDiarmid, T., Ziskin, M., & Michlovitz, S. L. (1996). Therapeutic ultrasound: Thermal agents in rehabilitation. F.A. Davis Company.
Moroni, R. M., Vieira, C. S., Ferriani, R. A., dos Reis, R. M., Nogueira, A. A., & Brito, L. G. O. (2015). Presentation and treatment of uterine leiomyoma in adolescence: a systematic review. BMC women's health, 15(1), 1-5. https://doi.org/10.1186/s12905-015-0162-9
O'Sullivan, A. K., Thompson, D., Chu, P., Lee, D. W., Stewart, E. A., & Weinstein, M. C. (2009). Cost-effectiveness of magnetic resonance guided focused ultrasound for the treatment of uterine fibroids. International Journal of Technology Assessment in Health Care, 25(1), 14-25. https://doi.org/10.1017/S0266462309090035
Ren, X. L., Zhou, X. D., Zhang, J., He, G. B., Han, Z. H., Zheng, M. J., & Wang, L. (2007). Extracorporeal ablation of uterine fibroids with high‐intensity focused ultrasound: imaging and histopathologic evaluation. Journal of Ultrasound in Medicine, 26(2), 201-212. https://doi.org/10.7863/jum.2007.26.2.201
Roebroeck, M. E., Dekker, J., & Oostendorp, R. A. (1998). The use of therapeutic ultrasound by physical therapists in Dutch primary health care. Physical Therapy, 78(5), 470-478. https://doi.org/10.1093/ptj/78.5.470
Schabrun, S., Walker, H., & Chipchase, L. (2008). How accurate are therapeutic ultrasound machines? Hong Kong Physiotherapy Journal, 26(1), 39-44.
Stewart, E. A., Nicholson, W. K., Bradley, L., & Borah, B. J. (2013). The burden of uterine fibroids for African-American women: results of a national survey. Journal of Women's Health, 22(10), 807-816. https://doi.org/10.1089/jwh.2013.4334
Sukhorukov, H. (2007). Multifunction systems for biotechnology. Biotechnology Journal, 25(3), 93–98.
Ter Haar, G. (2007). Therapeutic applications of ultrasound. Progress in Biophysics and Molecular Biology, 93(1-3), 111-129. https://doi.org/10.1016/j.pbiomolbio.2006.07.005
Vashisht, A., Studd, J. W. W., Carey, A. H., McCall, J., Burn, P. R., Healy, J. C., & Smith, J. R. (2000). Fibroid embolisation: a technique not without significant complications. BJOG: An International Journal of Obstetrics & Gynaecology, 107(9), 1166-1170. https://doi.org/10.1111/j.1471-0528.2000.tb11119.x
Wallach, E. E., & Vlahos, N. F. (2004). Uterine myomas: an overview of development, clinical features, and management. Obstetrics & Gynecology, 104(2), 393-406. https://doi.org/10.1097/01.AOG.0000136079.62513.39
Wise, L. A., Radin, R. G., Palmer, J. R., Kumanyika, S. K., & Rosenberg, L. (2010). A prospective study of dairy intake and risk of uterine leiomyomata. American Journal of Epidemiology, 171(2), 221-232. https://doi.org/10.1093/aje/kwp355
Zowall, H., Cairns, J. A., Brewer, C., Lamping, D. L., Gedroyc, W. M. W., & Regan, L. (2008). Cost‐effectiveness of magnetic resonance‐guided focused ultrasound surgery for treatment of uterine fibroids. An International Journal of Obstetrics & Gynaecology, 115(5), 653-662.
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