Effects of high-intensity interval training on hemoglobin levels and oxygen saturation in healthy males

Authors

  • Slamet Raharjo Department of Sport Science, Faculty of Sport Science, Universitas Negeri Malang, Indonesia.
  • Maharani Fatima Gandasari Study Program of Sports Coaching Education, Faculty of Teacher Training and Education, Universitas Tanjungpura, Indonesia.
  • Olivia Andiana Department of Sport Science, Faculty of Sport Science, Universitas Negeri Malang, Indonesia.
  • Pelana Ramdan Department of Sport Science, Faculty of Sport Science, Universitas Negeri Jakarta, Indonesia.
  • Raja Mohammed Firhad Raja Azidin Faculty of Sports Science and Recreation, Universitas Teknologi MARA, Malaysia.
DOI: https://doi.org/10.6018/sportk.565221
Keywords: HIIT, Hemoglobin, Oxygen Saturation, Heart Rate, Healthy Males

Abstract

The aim of the study was to examine the effects of high-intensity interval training (HIIT) with high-intensity continuous training (HICT) on increasing hemoglobin levels and oxygen saturation in healthy men. This study used a quasi-experiment with a pre-test-post-test group design. A total of 40 healthy male adolescents, aged 19-22 years, with a body mass index (BMI) of 20-24 kg/m2 and normal blood pressure, were recruited from university students and given HIIT and HICT intervention programs for 30 minutes. Hemoglobin levels were measured using Mission® Hemoglobin Test Strips, while oxygen saturation (SpO₂) and heart rate (HR) were measured using an Oxyone Pulse Oximeter. The mean pre-HIIT and HICT hemoglobin levels were 16.86 ± 1.39 vs 16.34 ± 1.28 g/dL (p = 0.221), and post-intervention were 18.36 ± 0.96 vs 15.41 ± 1.55 g/dL (p = 0.000). SpO₂ pre-values between HIIT and HICT were 96.75 ± 1.37 vs 96.45 ± 1.36 % (p = 0.491), and post-values were 97.85 ± 1.23 vs 96.15 ± 1.35 % (p = 0.000). HR pre-values between HIIT and HICT were 71.40 ± 7.03 vs 71.20 ± 7.98 bpm (p = 0.933), and post-values were 81.25 ± 11.79 vs 97.05 ± 5.54 bpm (p = 0.000). The study concluded that HIIT was effective in increasing hemoglobin and SpO2 levels as an indicator of cardiorespiratory fitness compared to HICT. Furthermore, HIIT was also found to be more effective at speeding up HR recovery.

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Published
10-10-2025
How to Cite
Raharjo, S., Gandasari, M. F., Andiana, O., Ramdan, P., & Azidin, R. M. F. R. (2025). Effects of high-intensity interval training on hemoglobin levels and oxygen saturation in healthy males. SPORT TK-EuroAmerican Journal of Sport Sciences, 14, 92. https://doi.org/10.6018/sportk.565221
Issue
Vol. 14 (2025)
Section
Articles

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The works and papers that are published in this Journal are subject to the following terms:

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© Servicio de Publicaciones, Universidad de Murcia, 2013

2. The papers and works are to be published in the digital edition of the Journal under the license Creative Commons Reconocimiento-No Comercial-Sin Obra Derivada 3.0 España (legal text). The copying, using, spreading, transmitting and publicly displaying of the papers, works or publication are permitted as long as: i) the authors and original sources (Journal, publisher and URL of the publication) are quoted; ii) it is not used for commercial benefit; iii) the existence and specifications of this users license are mentioned.

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