SEPARACIÓN DE SOLUTOS NO ENCAPSULADOS EN LIPQSOMAS. COMPARACIÓN DE LA FILTRACIÓN EN GEL CON LA CENTRIFUGACIÓN EN MINICOLUMNAS DE GEL
Abstract
We describe the separation of non-trapped material from a liposome preparation, both by gel´filtration and by centrifugation in Sephadex G-50 minicolumns. Both methods are compared according to different criteria: % of phospholipid recovery, sample dilution and calculation of th trapped volume for three different types of vesicles: multilamellar liposomes (MLV), sonicated unilamellar liposomes (SUV) and large unilamellar liposomes (LUV). The results obtained for the two procedures are similar, but there are several points wich favor to the centrifugation in gel minicolumns, esentially, the method is considerable faster, there is no sample dilution, small sample volumen can be processed and several samples can be processed at the same time. The values for the trapped volume measured by the two methods are similar and they are in good agreement with those mentioned in the literature.Downloads
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