Goat model for experimental mycoplasma contamination of semen: preliminary results

Authors

  • A. Gómez Martín
  • L. A. Vieira
  • A. De Ondiz
  • A. Paterna
  • J. Gadea
  • C. De la Fe
DOI: https://doi.org/10.6018/j/188791
Keywords: semen, experimental inoculation model, PPLO medium, mycoplasmas

Abstract

The presence of Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri in semen samples taken from asymptomatic bucks placed in artificial insemination centres has been confirmed in the last years. The ability of Mycoplasma spp. to cause adverse effects on sperm quality has been also demonstrated in several animal species including humans. In this sense, the risk of venereal transmission and the effects of mycoplasmas on sperm quality could affect goat breed improvement programs based on artificial insemination. The present study was conducted to develop an experimental model useful to study the effect of Mycoplasma spp. in goat semen. We evaluated the viability and motility of seminal doses maintained during two hours at 37 oC in semen samples previously kept under two temperature conditions (4-5 °C or 15-16 oC). The effect of PPLO medium in sperm motility, in which Mycoplasma spp. inocula are prepared, was also studied. Motility results registered in semen samples incubated at 37 oC during 120 minutes are better in seminal doses kept at 4-5 oC. PPLO medium had no significant effect on live or motile spermatozoa percentages registered after 150 minutes. Sperm viability and motility values (total and progressive) higher than 50% during the 150 and 60 minutes of incubation respectively were obtained using this medium. Overall, the present model is useful to conduct experimental contamination of goat semen doses with Mycoplasma spp. in order to evaluate its effect on spermatic viability and motility.

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How to Cite
Gómez Martín A., Vieira, L. A., De Ondiz, A., Paterna, A., Gadea, J., & De la Fe, C. (2012). Goat model for experimental mycoplasma contamination of semen: preliminary results. Anales de Veterinaria de Murcia, 28, 129–139. https://doi.org/10.6018/j/188791
Issue
Vol. 28 (2012)
Section
Artículos originales

 

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