Comparative value of microscopy, serology and real time pcr in the diagnosis of asymptomatic canine Leishmania infantum infection

Authors

  • J. Risueño
  • E. Bermejo
  • C. I. Muñoz García
  • I. Chitimia
  • L. del Río
  • J. D. García Martínez
  • E. Goyena
  • R. Fisa
  • C. Riera
  • P. Jiménez Montalbán
  • A. Martínez Ramírez
  • J. M. Meseguer Meseguer
  • L. Murcia
  • M. Segovia
  • E. Berriatua
DOI: https://doi.org/10.6018/j/188701
Keywords: cytology, ELISA, Leishmania infantum, optical microscopy, PCR

Abstract

The sensitivity (SE) of cytological examination of spleen and lymphnode smears by optical microscopy (OM), antibody-ELISA (enzyme-linked immunosorbent assays) and real-time (rt) PCR (polymerase chain reaction), for diagnosing asymptomatic canine Leishmania infantum infection was investigated in 110 apparently healthy dogs from southeast Spain. The percentage of OM, ELISA and rtPCR positive dogs were 2% (2/110), 27% (26/97) y 67% (39/58), respectively, although the percentage of rtPCR-positive dogs were 35-41% in individual tissues and 9% in blood. The estimated SE (95% confidence interval) of OM relative to the rtPCR and ELISA tests was 5% (0-12) and 8% (0-18), respectively. Results confirm that most apparently healthy dogs from L. infantum endemic areas are infected, that approximately only one third of these infected dogs develop antibodies and that very few have parasite loads that are high enough to allow detection by OM. As a result, the degree of agreement between rtPCR, ELISA and OM for L. infantum diagnosis in subclinnically infected dogs is low.

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How to Cite
Risueño, J., Bermejo, E., Muñoz García, C. I., Chitimia, I., del Río, L., García Martínez, J. D., … Berriatua, E. (2012). Comparative value of microscopy, serology and real time pcr in the diagnosis of asymptomatic canine Leishmania infantum infection. Anales de Veterinaria de Murcia, 28, 35–43. https://doi.org/10.6018/j/188701
Issue
Vol. 28 (2012)
Section
Artículos originales

 

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