Testicular parameters and morphological characteristic of epididymal spermatozoids collected postmortem in the bullfight
Abstract
In the present work has been evaluated testicular parameters and morphological characteristic different of epididymal spermatozoids collected postmortem in the bullfight as age and weigh animal, scrotal circumference, weigh and long testicular and spermatic variables. Twelve both testicles of bullfight coming from two farms of Domecq has been analyzed. After arrived of bullfight to slaughterhouse the scrotal circumference was measured and the testicles extracted and preserved to 5o C of temperature after yours identification. Afterwards the weigh and measures of each testicle was realized in the laboratory. The epididymal tail was dissectioned for to collect the sample. The spermatic volume and concentration and motility were measured. For last extension of eosin-nigrosin stamp were realized and life and dead spermatozoids, usual and unusual cells and usual acrosomes were determined. Before to stamp, the sample collected of each testicle was diluted 1:2 proportion with commercial diluted (Steridyl®) both moderating to 37oC. In order to study the sperm characteristics and testicular parameters of bullfighting coming from two farms, was a completely random arrangement design 2 x 2 factorial (two farms and two testes). The results show that there are significant differences (p < 0,05), testicular weight between livestock 1 of 467,33±17,89 and livestock 2 of 500,33±16,23. For variable scrotal circumference, there are significant differences (p < 0.01) between livestock 1 of 33, 5±0, 55 and livestock 36,5±1,52; finding the same significant difference for the weight of the right and left testicle between the first breeding of 295±4, 56, 293, 5±4, 41, and the second in 323, 17±14, 61 and 321, 12±14, 93 respectively. For the other variables evaluated, sperm concentration, individual motility, alive and dead, morfoanomalias and normal acrosomas, no statistically significant differences were found (p < 0,05). In general, it was found that in the variables that were statistically significant differences, always presented between farms and did not nothing to the testicle effect.Downloads
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