Optimization of conditions for the production of laccase by Trametes villosa (Sw.) Kreisel and its application in the bio-treatment of sugar cane vinasse

Authors

  • Rosa Elena Caballero Facultad de Ciencias Naturales y Exactas, Universidad Autónoma de Chiriquí, 0427, Chiriquí, Panamá
  • Víctor Jiménez Facultad de Ciencias Naturales y Exactas, Universidad Autónoma de Chiriquí, 0427, Chiriquí, Panamá
  • Mónica Miranda Facultad de Ciencias Naturales y Exactas, Universidad Autónoma de Chiriquí, 0427, Chiriquí, Panamá
  • Dalys Rovira Facultad de Ciencias Naturales y Exactas, Universidad Autónoma de Chiriquí, 0427, Chiriquí, Panamá
  • Pedro González Facultad de Ciencias Naturales y Exactas, Universidad Autónoma de Chiriquí, 0427, Chiriquí, Panamá
  • Juana Ramos Chue de Pérez Facultad de Ciencias y Tecnología, Universidad Tecnológica de Panamá, 0819-07289, El Dorado, Panamá
DOI: https://doi.org/10.6018/analesbio.43.03
Keywords: Residue, Organic load, Box-Behnken design

Abstract

Selected conditions for laccase production by Trametes villosa were optimized by surface response methodology: C:N ratio, Cu+2 level (mM), and fermentation time. The crude extract was inoculated on 25% (v/v) diluted sugarcane vinasse and some residual water quality parameters were evaluated. A 2.86-fold increase in the enzymatic activity was obtained upon optimization. Reduction of COD, phenol and color values on the treated vinasse were 46.33%, 73.98% and 52.87% respectively. pH increased from 6.50 to 6.57 and laccase enzymatic activity in vinasse media increased considerably. The advantage of surface respond methodologies for optimization of fermentation conditions is confirmed.

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Author Biography

Rosa Elena Caballero, Facultad de Ciencias Naturales y Exactas, Universidad Autónoma de Chiriquí, 0427, Chiriquí, Panamá

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Published
16-02-2021
How to Cite
Caballero, R. E., Jiménez, V. ., Miranda, M. ., Rovira, D., González, P., & Ramos Chue de Pérez, J. (2021). Optimization of conditions for the production of laccase by Trametes villosa (Sw.) Kreisel and its application in the bio-treatment of sugar cane vinasse. Anales de Biología, (43), 27–37. https://doi.org/10.6018/analesbio.43.03
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