Optimization of conditions for the production of laccase by Trametes villosa (Sw.) Kreisel and its application in the bio-treatment of sugar cane vinasse
Abstract
Selected conditions for laccase production by Trametes villosa were optimized by surface response methodology: C:N ratio, Cu+2 level (mM), and fermentation time. The crude extract was inoculated on 25% (v/v) diluted sugarcane vinasse and some residual water quality parameters were evaluated. A 2.86-fold increase in the enzymatic activity was obtained upon optimization. Reduction of COD, phenol and color values on the treated vinasse were 46.33%, 73.98% and 52.87% respectively. pH increased from 6.50 to 6.57 and laccase enzymatic activity in vinasse media increased considerably. The advantage of surface respond methodologies for optimization of fermentation conditions is confirmed.
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Author Biography
Rosa Elena Caballero, Facultad de Ciencias Naturales y Exactas, Universidad Autónoma de Chiriquí, 0427, Chiriquí, Panamá
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