UTILITY OF ACUTE PHASE PROTEINS IN CANINE CEREBROSPINAL FLUID FOR THE DIAGNOSIS OF MENINGOENCEPHALOMYELITIS OF UNKNOWN ORIGIN
Abstract
The immune system facilitates the defense of living beings, which is triggered through the acute phase response, generating acute phase proteins. These proteins, measured in serum, are useful. The use of biomarkers for the early diagnosis of inflammatory diseases of the central nervous system (CNS) is booming in human medicine. In the case of meningoencephalomyelitis of unknown origin (MOD), there are no studies with relevant information capable of solving the major drawback of not being able to obtain a definitive diagnosis, except postmortem. The measurement of acute phase proteins, such as C-reactive protein (CRP) and haptoglobin in cerebrospinal fluid (CSF) could help to solve this problem. The aim of this research is to assess whether these proteins can be detected in CSF using an automated biochemical analyzer and to study the existence of alterations in MOD animals. For this purpose, the animals included in the study were divided into a control group, composed of dogs without MOD, and a problem group, subdivided into dogs with MOD and dogs with MOD treated with corticoids. The Olympus AU600® analyzer was used to measure these parameters. To perform the comparative study between groups, the non-parametric Kruskal-Wallis test was applied, followed by Dunn's test for multiple comparisons. CRP did not show statistically significant variations, while haptoglobin could not be detected in CSF, so its measurement should be evaluated with more sensitive techniques that allow its quantification.
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