EFFECT OF TEMPERATURE ON THE MICROBIOTA OF THE EDIBLE INSECT ACHETA DOMESTICUS
Abstract
The insect microbiota performs essential functions in their metabolism, contributing to the proper functioning of the immune system and favoring digestion. The cricket species Acheta domesticus has recently been authorized for human consumption. The massive production of this species implies large energy costs in the breeding area, so variations in room temperature could mean significant savings in production costs. This study aims to analyze the influence of temperature on the composition of the intestinal microbiota, considering the hypothesis that changes in temperature affect the microbiota of ectothermic insects. For this, two groups of crickets were selected in separate boxes and subjected to temperatures of 20 ºC and 30 ºC, respectively. Subsequently, their intestines were removed and their bacterial populations were quantified using metagenomic techniques. The results obtained showed that the most abundant taxa hardly varied in all samples. Abundance relatives of Bacteria belonging to the phylum Firmicutes was 31.5 ± 2.06%, in Bacteroidetes 24.25 ± 3.89% and in Proteobacteria 44 ± 4.85%. The only sample showing difference was that reared at 30ºC, in which 0.8% of Actinobacteria was detected. In conclusion, it could be said that the rearing temperature of cricket farms has slightly influenced the composition of the intestinal microbiota, therefore the initial hypothesis is accepted. Incidentally, high mortality has been observed in the sample kept at 30ºC, suggesting that this factor should be carefully considered in massive rearing of crickets.
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References
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