ESTUDIO ANATOMOPATOLÓGICO Y MASTOCITOS EN HÍGADOS DE TERNERO DECOMISADOS EN MATADERO
Abstract
This study research the anatomopathological injuries and the number of mast cells in 51 confiscated calf livers in a major slaughterhouse in the south-east of Spain. The microscopic analysis was realized with hematoxylin-eosin (H-E) and Masson's Trichrome (CRT) stains. Furthermore, the number of mast cells was determined based on their pathologies, using toluidine blue staining, counting in 10 fields of 348 x 263 µm at 40x in each of the samples and with the SPSS statistical program and the Kolmogorov-Smirnov and Mann-Whitney tests compared with 5 control livers. The pathological lesions of confiscation were: abscesses (47%), cholangiohepatitis (31%), fibrosis (8%), steatosis (6%), atrophy (2%), necrosis (2%), congestion (2%) and cyst (2%). Classifying into inflammatory lesions (49%), parasitic lesions (33%) and degenerative-metabolic lesions (18%). The results conclude that there are significant differences in the number of mast cells in livers with cholangiohepatitis (p 0,001) compared with control livers and there are no significant differences in livers with abscesses (p 0,227). When classifying the pathological alterations by families, there are no significant differences in the inflammatory alterations when compared them with the control group and there are differences in the parasitic alterations. Degenerative alterations show significative differences, but the number of samples collected is scarce.
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Amachawadi, R. G., Tom, W. A., Hays, M. P., Fernando, S. C., Hardwidge, P. R., y Nagaraja, T. G. (2021). Bacterial community analysis of purulent material from liver abscesses of crossbred cattle and Holstein steers fed finishing diets with or without tylosin. Journal of Animal Science, 99(4). doi:10.1093/jas/skab076
Ascarza, Y. P. C. (2013). Enfermedades parasitarias causantes de condena de vísceras y pérdida económica, en rumiantes del camal municipal de Abancay, septiembre de 2012. Edu.pe website: http://repositorio.unamba.edu.pe/bitstream/handle/UNAMBA/442/T_0050.pdf?sequence=1&isAllowed=y
Brown, D. L., Van Wettere, A. J., y Cullen, A. J. M. (2016). Hepatobiliary System and Exocrine Pancreas. En Elsevier (Ed.), Pathologic Basis of Veterinary Disease. Mosby.
Cedeño, A., Martínez, G., y Cilima. (2012). Principales causas de decomiso de vísceras rojas en bovinos en el frigorífico del municipio de pasto. https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&ved=2ahUKEwjo7Iftlez4AhURiv0HHRgoD3QQFnoECB0QAQ&url=https%3A%2F%2Frevistas.udenar.edu.co%2Findex.php%2Frevip%2Farticle%2Fdownload%2F380%2F394%2F&usg=AOvVaw3ZhcI3I35DfHv2TMJCknJ6
Dudeck, A., Köberle, M., Goldman, O., Meyer, N., Dudeck, J., Lemmens, S., Rodhe, M., González, N., Dietze-Schwonberg, K., Orinska, Z., Medina, E., Hendrix, S., Metz, M., Zenclussen, A., Von, E., y Biodermann, T. (2018). Mast cells as protectors of health. Jacionline.org website: https://www.jacionline.org/article/S0091-6749(18)31605-1/fulltext
Edwards, J. F. (2016). Patología hepática bovina de la alfa a la omega. XLIV Jornadas Uruguayas de Buiatria website: https://bibliotecadigital.fvet.edu.uy/bitstream/handle/123456789/1366/JB2016_56-58.pdf?sequence=1&isAllowed=y
Faccin, T. C., Kommers, G. D., Galiza, G. J. N. de, Pupin, R. C., Madureira, R. C., y Lemos, R. A. A. de. (2016). Chronic liver disease in cattle associated with ingestion of Brachiaria spp. Ciencia rural, 46(11), 2036–2042. doi:10.1590/0103-8478cr20160297
Franceschini, B., Russo, C., Dioguardi, N., y Grizzi, F. (2007). Increased liver mast cell recruitment in patients with chronic C virus-related hepatitis and histologically documented steatosis. Journal of Viral Hepatitis, 14(8), 549–555. doi:10.1111/j.1365-2893.2007.00859.x
Horowitz, J. C., y Thannickal, V. J. (2019). Mechanisms for the resolution of organ fibrosis. Physiology (Bethesda, Md.), 34(1), 43–55. doi:10.1152/physiol.00033.2018
Huntley, J. F., Patterson, M., Mackellar, A., Jackson, F., Stevenson, L. M., y Coop, R. L. (1995). A comparison of the mast cell and eosinophil responses of sheep and goats to gastrointestinal nematode infections. Research in Veterinary Science, 58(1), 5–10. doi:10.1016/0034-5288(95)90080-2
Izquierdo, N (2015). El decomiso de vísceras en los mataderos. https://www.researchgate.net/publication/313030878_El_decomiso_de_visceras_en_los_mataderos
Koruk, S. T., Ozardali, I., Dincoglu, D., y Bitiren, M. (2011). Increased liver mast cells in patients with chronic hepatitis C. Indian Journal of Pathology & Microbiology, 54(4), 736–740. doi:10.4103/0377-4929.91510
Lázaro, F. R., Lázaro, D. R., Abuín, C. M. F., Calleja, C. A., Escámez, P. F., y González, E. (2021). Informe del Comité Científico de la Agencia Española de Seguridad Alimentaria y Nutrición (AESAN) sobre los casos en los que procede realizar procedimientos adicionales a la inspección post mortem de rutina en matadero. AESAN website: https://www.aesan.gob.es/AECOSAN/docs/documentos/seguridad_alimentaria/evaluacion_ries gos/informes_comite/INSPECCION_POST_MORTEM.pdf
Martínez, G., Suarez, V. H., y Mirabal, R. (2017). SA 16 efecto de la disminución de fibra en la dieta de vacas lecheras preparto sobre la salud animal y la performance productiva. 40° Congreso Argentino de Producción Animal. Gob.ar. website: https://inta.gob.ar/sites/default/files/salud_animal_aapa.pdf
MAPA. Subdirección General de Producciones Ganaderas y Cinegéticas. Dirección General de Producciones y Mercados Agrarios. Ministerio de Agricultura, P. y. A. (2021). Caracterización del sector vacuno de carne en España. Gob.es website: https://www.mapa.gob.es/es/ganaderia/temas/produccion-y-mercados- ganaderos/caracterizacionsectorvacunodecarne_julio2021_pub_tcm30-553721.pdf
Maurer, M., Köberle, M., y Metz, M. (2019). Mast cells: Promoters of health and modulators of disease. Jacionline.org website: https://www.jacionline.org/article/S0091-6749(19)30284-2/fulltext
Pinnell, L. J., Whitlow, C. W., Huebner, K. L., Bryant, T. C., Martin, J., Belk, K. E., y Morley, P. S. (2022). Not all liver abscesses are created equal: The impact of tylosin and antibiotic alternatives on bovine liver abscess microbial communities and a first look at Bacteroidetes-dominated communities. Frontiers in Microbiology, 13, 882419. doi:10.3389/fmicb.2022.882419
Roger Kely, W. (2002). Enfermedad del hígado en grande y pequeños rumiantes. Congreso, X; De Buiatría, Latinoamericano. XXX Jornadas Uruguayas de Buiatría website: https://bibliotecadigital.fvet.edu.uy/bitstream/handle/123456789/456/JB2002_1-6.pdf?sequence=1&isAllowed=y
Sánchez, P. (2013). Diagnóstico de procesos patológicos en ungulados domésticos sacrificados en matadero en el sureste de España. [Tesis doctoral, Facultad de veterinaria]. Universidad de Murcia.
Weiskirchen, R., Meurer, S. K., Liedtke, C., y Huber, M. (2019). Mast cells in liver fibrogenesis. Cells (Basel, Switzerland), 8(11), 1429. doi:10.3390/cells8111429
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