IMMUNOCYTOCHEMICAL AND ULTRASTRUCTURAL STUDY OF GONADOTROPE CELLS OF LAMB (<i>OVIS ARIES</i> L.).
Abstract
We have studied the structure and ultrastructure of adenohypophyseal of 6 males and 6 females 01' sheep belonging to the Segureña breed, aged 3 months and weigthing about 24 kg. Routine staining and histochemical methods (Herlant tetracrome. PAS, PAS-O, AA-PAS-OG and Cleveland-Wolfe) are compared with avidine-biotine-complex (ABC). Ultrastructrual identification of cells is based on comparisons between ultrathin sections and semithin sections labelled by means of the peroxidase-antiperoxidase lechnique (PAP). Antisera FSHh and LHh were utilized previously adsworbed wilh FSH and LH hormones. Gonadotrope LH cells are a 7.5-10% and FSH cells are a 0.1% of the total of cells and appear distributed in the anterior and dorso-caudal region after a sagital section. These cells are characterized by large lisosomes. a single type of secretory granules (250 nm) and different appearance of granular endoplasmic reticulum.Downloads
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