In vitro effect of crude extracts from eight Algerian steppe plants on mycelial growth and sporulation of Ascochyta pisi Lib.

Authors

  • Ammar Tiaiba Department of Agricultural Sciences, Faculty of Sciences, University of M’Sila, BP 166 Echbilia, 28000 M'Sila, Algeria.
  • Malika Derbali Department of Natural and Life Sciences, Faculty of Sciences, University of M’Sila, BP 166 Echbilia, 28000 M'Sila, Algeria.
  • Boubekeur Seddik Bendahmane Department of Agronomy, University of Mostaganem, Mostaganem, Algeria.
DOI: https://doi.org/10.6018/analesbio.40.13
Keywords: Ascochyta blight, Pea, Growth inhibition, Sporulation, Steppe plants, Crude extract

Abstract

This work consists of studying the effect of crude extracts of eight plants growing in the Algerian steppe against Ascochyta pisi Lib., one of the members of the Ascochyta complex causing the Ascochyta blight of pea, with the aim of substituting them for chemical fungicides recognized for their harmfulness to the environment and public health. For this purpose, an in-vitro biocontrol test of the growth and sporulation of A. pisi was achieved by adding to its cultural medium a crude plant extracts or their sub-fractions. At the end of this trial, we found that the extracts of Artemisia alba Turra, Lycium arabicum Schweinf. Ex Boiss. and Peganum harmala Linn. recorded the best growth inhibition rates of pathogenic whereas, Artemisia campestris L. is more effective against its sporulation.

Downloads

Download data is not yet available.

Author Biography

Boubekeur Seddik Bendahmane, Department of Agronomy, University of Mostaganem, Mostaganem, Algeria.

Plant Protection Laboratory, University of Mostaganem, Mostaganem, Algeria

References

Ahmed H, Chang KF, Hwang SF, Fu H, Zhou Q, Strelkov S, Conner R & Gossen B. 2015. Morphological cha­racterization of fungi associated with the ascochyta blight complex and pathogenic Variability of Mycosphaerella pinodes on field pea in central Alberta. The Crop Journal 3: 10-18.

Barilli E, Cobos MJ & Rubiales D. 2016. Clarification on Host Range of Didymella pinodes the Causal Agent of Pea Ascochyta Blight. Frontiers in Plant Science 7:592 [16].

Bowen J K, Lewis BG & Matthews P. 1997. Discovery of the teleomorph of Phoma medicaginis var. pinodella in culture. Mycological Research 101(1): 80-84.

Dalili A, Bakhtiari S, Barari H & Aldaghi M. 2015. Effect of some fungicides against the growth inhibition of Sclerotinia sclerotiorum mycelial compatibility groups. Journal of Plant Protection Research 55 (4): 354-361.

Davidson JA, Hartley D, Priest M, Krysinska-Kaczmarek M, Herdina-McKay A & Scott ES. 2009. A new species of Phoma causes ascochyta blight symptoms on field peas (Pisum sativum) in South Australia. Mycologia 101: 120-128.

El Guilli M, Achbani E, Fahad K & Jijakli H. 2009. Biopesticides: Alternatives à la lutte chimique. Symposium International «Agriculture durable en région méditerranéenne», 14-16 Mai 2009, Rabat Maroc.

Fondevilla S, Cubero JL & Rubiales D. 2007. Inheritance of resistance to Mycosphaerella pinodes in two wild accessions of Pisum. European Journal of Plant Pathology 119: 53–58.

Jones WP & Kinghorn AD. 2006. Extraction of Plant Secondary Metabolites. From: Methods in Biotechnology, Natural Products Isolation. Totowa: Humana Press Inc.

Mahlo SM, McGaw LJ & Eloff JN. 2010. Antifungal acti­vity of leaf extracts from South African trees against plant pathogens. Crop Protection 29: 1529-1533.

Ogbebor N & Adekunle AT. 2005. Inhibition of conidial germination and mycelial growth of Corynespora cassiicola (Berk & Curt) of rubber (Hevea brasiliensis muell. Arg.) using extracts of some plants. African Journal of Biotechnology 4(9): 996-1000.

Onfroy C, Baranger A & Tivoli B. 2007. Biotic factors affecting the expression of partial resistance in pea to ascochyta blight in a detached stipule assay. European Journal of Plant Pathology 119: 13-27.

Pretorius JC, Craven P & Van der Watt E. 2002. In vivo control of Mycosphaerella pinodes on pea leaves by a crude bulb extract of Eucomis autumnalis. Annals of Applied Biology 141: 125-131.

Schoeny A, Jumel S, Rouault F, Le May C & Tivoli B. 2007. Assessment of airborne primary inoculum availability and modelling of disease onset of ascochyta blight in field peas. European Journal of Plant Pathology 119: 87-97

Tadja A, Youcef Benkada M, Rickauer M, Bendahmane BS & Benkhelifa M. 2009. Characterization of Ascochyta as Pathological Species of Pea (Pisum sativum L.) at the North-West of Algeria. Journal of Agronomy 8(3): 100-106.

Tivoli B & Banniza S. 2007. Comparison of the epide­miology of ascochyta blights on grain legumes. European Journal of Plant Pathology 119: 59-76.

Tegegne G, Pretorius JC & Swart WJ. 2008. Antifungal properties of Agapanthus africanus L. extracts against plant pathogens. Crop Protection 27: 1052-1060.

Tran HS, You MP, Lanoiselet V, Khan T N & Barbetti M J. 2014. First Report of Phoma glomerata Asso­ciated with the Ascochyta Blight Complex on Field Pea (Pisum sativum) in Australia. Plant disease 98 (3): 427-427.

Published
30-11-2018
How to Cite
Tiaiba, A., Derbali, M., & Bendahmane, B. S. (2018). In vitro effect of crude extracts from eight Algerian steppe plants on mycelial growth and sporulation of Ascochyta pisi Lib. Anales de Biología, (40), 115–120. https://doi.org/10.6018/analesbio.40.13
Issue
Section
Articles