Morphoanatomical and histochemical study of Ipomoea hederifolia L. (Convolvulaceae)
Abstract
Ipomoea hederifolia L. is a herbaceous vine native to the tropical Americas with important medicinal properties. Was realized a pharmacobotanical study of the leaves and stems of this species, performing macroscopic and microscopic morphodiagnoses and histochemical tests. Anatomical characteristics typical of the family Convolvulaceae were found. However, the epidermis and its appendages (e.g. striated cuticle and peltate trichomes) and the anatomy of the petiole and the stem presented relevant characters for the taxonomic recognition of the species. Histochemical tests evidenced the presence of lignin and cutin and positive reactions for starch, phenolic compounds, and proteins. The anatomy and the histochemical tests indicated a set of characteristics relevant to the pharmacobotanical characterization of I. hederifolia, expanding our knowledge of the species and providing subsidies for the quality control of its vegetal products.
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Abba HM, Abdullahi A & Yuguda UA. 2018. Leaf epidermal anatomy of Ipomoea carnea Jacq sampled from selected areas in Gombe State, Nigeria. Bayero Journal of Pure and Applied Sciences 11(1): 148-154. https://doi.org/10.4314/bajopas.v11i1. 26
Agra MF, Nurit-Silva K, Basílio IJLD, Freitas PF & Barbosa-Filho JM. 2008. Survey of medicinal plants used in the region Northeast of Brazil. Revista Brasileira de Farmacognosia 18: 472-508.
Araújo ND, Coelho VPM, Ventrella M & Agra MF. 2013. Leaf anatomy and histochemistry of three species of Ficus sect. Americanae Supported by Light and Electron Microscopy. Microscopy and Microanalysis 20(1): 1-9. https://doi.org/10.1017/S143192761301 3743
Arruda RCO, Viglio NSF & Barros AAMD. 2009. Anatomia foliar de halófitas e psamófilas reptantes ocorrentes na restinga de Ipitangas, Saquarema, Rio de Janeiro, Brasil. Rodriguésia 60 (2): 333-352.
Ashfaq S, Ahmad M, Zafar M, Sultana S, Bahadur S, Ullah, F & Nazish M. 2019. Foliar micromorphology of Convolvulaceous species with special emphasis on trichome diversity from the arid zone of Pakistan. Flora 255: 110-124. https://doi.org/10.1016/j.flora. 2019.04.007
Azania CAM, Hirata ACS & Azania AAPM. 2011. Biologia e manejo químico de corda de viola em cana-de-açúcar. Boletim Técnico IAC 209. Campinas: IAC.
Babu K, Dharishini MP, & Austin A. 2018. Studies on anatomy and phytochemical analysis of Ipomoea pes-tigridis L. Journal of Pharmacognosy and Phytochemistry 7 (1): 791-794.
Bandeira ÁNT, Bautista HP, Buril MT & Melo JIMD. 2019. Convolvulaceae no Parque Ecológico Engenheiro Ávidos, Alto Sertão Paraibano, Nordeste do Brasil. Rodriguésia 70: 2-18. https://doi.org/10. 1590/2175-7860201970026
Berlyn GP & Miksche JP. 1976. Botanical microtechinique and cytochemistry, Ames: Yowa State University Press.
Bolarinwa KA, Oyebanji OO & Olowokudejo JD. 2018. Comparative morphology of leaf epidermis in the genus Ipomoea (Convolvulaceae) in Southern Nigeria. Annals of West University of Timişoara, ser. Biology 21(1): 29-46.
Bowling AJ & Vaughn KC. 2009. Gelatinous fibers are widespread in coiling tendrils and twining vines. American Journal of Botany 96(4): 719-727. https://doi.org/10.3732/ajb.0800373
Brandão M & Gavilanes, ML. 1997. Uma nova ocorrência do gênero Ipomoea L. (Convolvulaceae) para Minas Gerais-1. Ipomoea hederifolia L. Daphne 7(4): 7-8.
Carlquist S & Hanson MA. 1991. Wood and Stem Anatomy of Convolvulaceae. Aliso: A Journal of Systematic and Evolutionary Botany 13 (1): 51-94. https:// 10.5642/aliso.19911301.03
Conceição GM, Silva DS & Rodrigues MS. 2014. Aspectos florísticos e ecológicos da família Convolvulaceae da área de proteção ambiental municipal do Inhamum, Caxias, Maranhão, Brasil. Brazilian Geographical Journal: Geosciences and Humanities Research Medium 5(2): 595-613.
Flora e Funga do Brasil, Jardim Botânico do Rio de Janeiro. 2020. Convolvulaceae Juss. Available in http://reflora.jbrj.gov.br/reflora/floradobrasil/FB93 (accessed on 1-II-2022).
Flora e Funga do Brasil, Jardim Botânico do Rio de Janeiro. 2020. Ipomoea L. .Available in http://reflora. jbrj.gov.br/reflora/floradobrasil/FB7021 (accessed on 1-II-2022).
Cutter EG. 1986. Anatomia Vegetal; Parte II - Órgãos - Experimentos e Interpretações. São Paulo: Roca Ltda.
Dan-Sheng C, You-Xiong D, Rui-Jun M, Hui Z & Ai-Na L. 2007. Anatomical structure of stem of Ipomoea cairica (Convolvulaceae). Plant Diversity 29(2): 189-192.
Delgado Júnior GC, Buril MT & Alves M. 2014. Convolvulaceae do Parque Nacional do Catimbau, Pernambuco, Brasil. Rodriguésia, 65 (2): 425-442. https://doi.org/10.1590/S2175-78602014000200008
Esau K. 1977. Anatomy of seed plants. New York: Jonh Wiley & Sons.
Fahn A. 1990. Plant anatomy. Oxford: Pergamon Press.
Folorunso AE. 2013. Taxonomic evaluation of fifteen species of Ipomoea L. (Convolvulaceae) from South-Western Nigeria using foliar micromorphological characters. Notulae Scientia Biologicae 5(2): 156-162. https://doi.org/10.15835/nsb529056
Garcia-Blanco H. 1972. Importância dos estudos ecológicos nos programas de controle das plantas daninhas. Biológico 38(10): 343-50.
Harris JG & Harris MW. 2001. Plant Identification Terminology: An illustrated glossary. Spring Lake: Spring Lake Publishing.
Jenett-Siems K, Kaloga M & Eich E.1993. Ipangulines, the first pyrrolizidine alkaloids from the Convolvulaceae. Phytochemistry 34(2): 437-440. https://doi. org/10.1016/0031-9422(93)80025-N
Jensen WA. 1962. Botanical histochemistry: principles and practice. San Francisco: WH, Freeman & Co.
Johansen DA. 1940. Plant microtechnique. New York: McGrawHill.
Khalifa AA, Mohamed AA, Ibrheim ZZ & Hamoda AMA. 2017. Macro and Micromorphology of the Leaves, Stems, Seeds and Fruits of Ipomoea eriocarpa (R. Br.) Growing in Egypt. Bulletin of Pharmaceutical Sciences. Assiut 40: 9-31. https://doi.org/10.21608/bfsa. 2017.63162
Kiill LHP & Ranga NT. 2003. Ecologia da polinização de Ipomoea asarifolia (Ders.) Roem. & Schult. (Convolvulaceae) na região semi-árida de Pernambuco. Acta Botanica Brasilica 17(3): 355-362.
Kissmann KG & Groth D. 1999. Plantas infestantes e nocivas. São Paulo: BASF.
Kraus JE & Arduin M. 1997. Manual básico de métodos em morfologia vegetal. Rio de Janeiro: EDUR.
Kuster VC, Silva LC, Meira RMSA & Azevedo AA. 2016. Glandular trichomes and laticifers in leaves of Ipomoea pes-caprae and I. imperati (Convolvulaceae) from coastal Restinga formation: Structure and histochemistry. Brazilian Journal of Botany 39(4): 1117-1125. https://doi.org/10.1007/s40415-016-0308-5
Lemos VDOT, Lucena EMPD, Bonilla OH & Edson-Chaves B. 2019. Ecological anatomy of Eugenia punicifolia (Kunth) DC. (Myrtaceae) in the restinga region, state of Ceará. Revista Brasileira de Fruticultura 41(6): 1-11. https://doi.org/10.1590/ 0100-29452019503
Lima APSL & Melo JIM. 2019. Ipomoea L. (Convolvulaceae) na mesorregião agreste do Estado da Paraíba, Nordeste brasileiro. Hoehnea 46: 1-21. https://doi.org/10.1590/2236-8906-43/2018
Lopes-Silva RF, Silva ALE, Santos EAV & Agra MDF. 2021. Leaflet blade epidermis and its taxonomic significance in 13 species of Bignonieae (Bignoniaceae) from Pico do Jabre, Paraíba, Northeast of Brazil. Botany 99(2): 75-90. https://doi.org/10.1139/ cjb-2020-0051
Lorenzi H. 2000. Plantas daninhas do Brasil, Terrestres, Aquáticas, Parasitas e Tóxicas. Nova Odessa: Instituto Plantarum.
Lowell C & Lucansky TW. 1986. Vegetative anatomy and morphology of Ipomoea hederifolia (Convolvulaceae). Bulletin of the Torrey Botanical Club 113(4): 382-397. https://doi.org/10.2307/2996431
Mandal S, Chodhury S & Chowdhury HR. 2015. Studies on Ipomoea cairica (L.) sweet-A promising ethnomedicinally important plant. Journal of Innovations in Pharmaceuticals and Biological Sciences 2(4): 378-395.
Martins FM, Lima JF, Mascarenhas AAS & Macedo TP 2012. Secretory structures of Ipomoea asarifolia: anatomy and histochemistry. Revista Brasileira de farmacognosia 22(1): 13-20. https://doi.org/10.1590/ S0102-695X2011005000162
Meena B & Santhi G. 2018. Phytochemical screening and in vitro hepatoprotective activity of Ipomoea obscura. World Journal of Pharmaceutical Research 7(3): 1623-1636. https://doi.org/10.20959/wjpr20183 -10996
Meira M, Silva EP, David JM & David JP. 2012. Review of the genus Ipomoea: traditional uses, chemistry and biological activities. Revista Brasileira de Farmacognosia, 22 (3): 682-713. https://doi.org/10. 1590/S0102-695X2012005000025
Metcalfe CR & Chalk L. 1950. Anatomy of the dicotyledons. London: Oxford University Press.
Monquero PA, Cury JC & Chistoffoleti P J. 2005. Controle pelo glyphosate e caracterização geral da superfície foliar de Commelina benghalensis, Ipomoea hederifolia, Richardia brasiliensis e Galinsoga parviflora. Planta daninha 23(1):123-132. https://doi.org/10.1590/S0100-83582005000100015
Moreira HJC & Bragança HBN. 2011. Manual de identificação de plantas infestantes: Hortifruti. Campinas: FMC Agricultural Products.
Moshobane MC, Winter P & Middleton L. 2022. Record of naturalized Ipomoea hederifolia (Linnaeus 1759) (Convolvulaceae), Scarlet morning-glory in South Africa. BioInvasions Records 11(1): 49-56. https://doi.org/10.3391/bir.2022.11.1.05
Muir CD. 2019. Is amphistomy an adaptation to high light? Optimality models of stomatal traits along light gradients. Integrative and comparative biology 59(3): 571-584. https://doi.org/10.1093/icb/icz085
Mukherjee D, Gupta A, Soni D & Jana G. K. 2011. Ipomoea fistulosa: An evaluation of its pharmacognostical and phytochemical profile. International Journal of Chemical and Analytical Science 2(12): 1270-1273.
Nakata PA. 2012. Plant calcium oxalate crystal formation, function, and its impact on human health. Frontiers in biology 7(3): 254-266. https://doi.org/10. 1007/s11515-012-1224-0
Neves MVM, Araújo ND, Oliveira EJ & Agra MF. 2016. Leaf and stem anatomy and histochemistry of Dalbergia ecastaphyllum. Pharmacognosy Journal 8: 557-564. https://doi.org/10.5530/pj.2016.6.7
Nilam R, Jyoti P & Sumitra C. 2018. Pharmacognostic and phytochemical studies of Ipomoea pes-caprae, an halophyte from Gujarat. Journal of Pharmacognosy and Phytochemistry 7(1): 11-18.
Nurit-Silva K & Agra MF. 2011. Leaf Epidermal Characters of Solanum Section Polytrichum (Solanaceae) as Taxonomic Evidence. Microscopy Research and Technique 74(12): 1186-91 https://doi.org/10.1002/jemt.21013
Nurit-Silva K, Costa-Silva R, Basílio IJLD & Agra MF. 2012. Leaf epidermal characters of Brazilian species of Solanum section Torva as taxonomic evidence. Botany, 90: 1-9. https://doi.org/10.1139/b2012-046
Olaranont Y, Stauffer FW, Traiperm P & Staples GW. 2018. Investigation of the black dots on leaves of Stictocardia species (Convolvulaceae) using anatomical and histochemical analyses. Flora 249: 133-142. https://doi.org/10.1016/j.flora.2018.10.007
Osorio N, Charry PA, Rios-Vásquez E & Castañeda-Gómez JF. 2018. Ácidos orgánicos constitutivos de las resinas glicosídicasde tres especies de Ipomoea (Convolvulaceae). Revista ION 31(1): 55-58. http://dx.doi.org/10.18273/revion.v31n1-2018009
Paes LS & Mendonça MS. 2008. Aspectos morfoanatômico de Bonamia ferruginea (Choisy) Hallier F. (Convolvulaceae). Revista Brasileira de Plantas Medicinais 10(4): 76-82.
Pandurangan A & Rana K. 2015. A mini review on chemistry and biology of Ipomoea hederifolia Linn. (Convolvulaceae). Global Journal of Pharmaceutical Education and Research 4(1-2): 23-25.
Patil VS, Rao, KS & Rajput KS. 2009. Development of intraxylary phloem and internal cambium in Ipomoea hederifolia (Convolvulaceae). Journal of the Torrey Botanical Society 136(4): 423-432.
Pegorini F, Maranho LT & Rocha LD. 2008. Organização estrutural das folhas de Baccharis dracunculifolia DC., Asteraceae. Revista Brasileira de Farmacognosia 89(3): 272-275.
Peiffer M, Tooker JF, Luthe DS & Felton GW. 2009. Plants on early alert: glandular trichomes as sensors for insect herbivores. New Phytologist 184(3): 644-656. https://doi.org/10.1111/j.1469-8137.2009.0300 2. x
Pereira LBS, Costa-Silva R, Felix LP & Agra MF. 2018. Leaf morphoanatomy of “mororó” (Bauhinia and Schnella, Fabaceae). Revista Brasileira de Farmacognosia 28(4): 383-392. https://doi.org/10. 1016/j.bjp.2018.04.012
Pereira ZV, Meira RMSA & Azevedo AA. 2003. Leaf morpho-anatomy of Palicourea longepedunculata Gardiner (Rubiaceae). Revista Árvore 27(6): 759-767. https://doi.org/10.1590/S0100-6762200300060 0002
Porto NM, Barros YLD, Basílio IJ & Agra MF. 2016. Microscopic and UV/Vis spectrophotometric characterization of Cissampelos pareira of Brazil and Africa. Revista Brasileira de Farmacognosia 26(2): 135-146. https://doi.org/10.1016/j.bjp.2015.10.006
Porwal O, Gupta S, Nanjan MJ & Singh A. 2015. Classical taxonomy studies of medicinally important Ipomoea leari. Ancient science of life 35(1): 34-41. https://doi.org/10.4103/0257-7941.165628
Prasanth B, Aleykutty NA & Harindran J. 2018. Pharmacognostic Studies on leaves and stems of Ipomoea sepiaria Roxb. International Journal of Pharmaceutical Sciences and Research, 9 (9): 3938-3943. https://doi.org/10.13040/IJPSR.0975-8232
Pulido-Salas MT. 1993. Plantas útiles para consumo familiar en la región de la frontera Mexico-Belice. Caribbean Journal of Science 29(3-4): 235-249.
Rajendran K, Srinivasan KK & Shirwaikar A. 2007. A. Pharmacognostical Identification of Stem and Root of Ipomoea quamoclit (Linn.). Natural Product Sciences 13(4): 273-278.
Santos D, Saraiva RVC, Ferraz TM, Arruda ECP & Buril MT. 2020. A threatened new species of Ipomoea (Convolvulaceae) from the Brazilian Cerrado revealed by morpho-anatomical analysis. PhytoKeys 151: 93-106. https://doi.org/10.3897/phytokeys.151. 49833
Santos EAV & Nurit-Silva K. 2015. Estudo anatômico dos órgãos vegetativos aéreos de Ipomoea triloba L. (Convolvulaceae). Revista Saúde & Ciência Online 4(3): 89- 93.
Santos EAV & Nurit-Silva K. 2018. Morfo-anatomia dos órgãos vegetativos de Ipomoea longeramosa Choisy (Convolvulaceae). Anais do III Congresso Nacional de Pesquisa em Ensino de Ciências III (CONAPESC), Campina Grande [12].
Sass JE. 1951. Botanical microtechnique. Iowa: State College Press.
Silva IAB, Kuva MA, Alves PLCA & Salgado TP. 2009. Interferência de uma comunidade de plantas daninhas com predominância de Ipomoea hederifolia na cana-soca. Planta daninha 27(2): 265-272. https://doi.org/10.1590/S0100-8358200900020 0008
Silva ML & Lemos JR. 2020. Aspectos anatômicos de plantas do semiárido. In: Morfoanatomia de plantas do semiárido (Lemos JR., ed.) São Paulo: Blucher Open Access, pp. 51-71.
Silva MVP. 2013. Eficiência e seletividade de herbicidas pré-emergentes aplicados sobre a palha na cultura da cana-de-açúcar. Rio Largo, Alagoas: Universidade Federal de Alagoas. Dissertação de Mestrado.
Srivastava D & Rauniyar N. 2020. Medicinal plants of genus Ipomoea: A glimpse of potential bioactive compounds of genus Ipomoea and its detail. London LAP LAMBERT Academic Publishing.
Stevens PF. Angiosperm Phylogeny Website, version 14. 2017. Available at: http://www.mobot.org/MOBOT/research/APweb/ (accessed on 1-II-2022).
Tamaio N, Braga JMA & Rajput KS. 2021. Development of successive cambia and structure of secondary xylem in the stems and roots of Distimake tuberosus (Convolvulaceae). Flora 279: 151814 [10]. https:// doi.org/10.1016/j.flora.2021.151814
Tayade SK & Patil DA. 2012. Foliar anatomy of some Uninvestigated species of Convolvulaceae. Life Sciences Leaflets 3: 75-86.
Tooulakou G, Giannopoulos A, Nikolopoulos D, Bresta P, Dotsika E, Orkoula MG & Karabourniotis G. 2016. Alarm photosynthesis: calcium oxalate crystals as an internal CO2 source in plants. Plant Physiology 171(4): 2577-2585. https://doi.org/10.1104/pp.16.00 111
Traiperm P, Chow J, Nopun P, Staples G & Swangpol SC. 2017. Identification among morphologically similar Argyreia (Convolvulaceae) based on leaf anatomy and phenetic analyses. Botanical studies 58(1): 1-14. https://doi.org/10.1186/s40529-017-017 8-6
Vidal BC. 1970. Dichroism in collagen bundles stained with xylidine Ponceau 2R. Annalytical Histochemistry 15 (4): 289-296.
Werker E. 2000. Trichome diversity and development. Advances in Botanical Research 31: 1-35. https://doi.org/10.1016/S0065-2296(00)31005-9
Wilkinson HP. 1979. The plant surface (mainly leaf). In: Anatomy of the Dicotyledons (Metcalfe CR & Chalk L, eds.). Oxford: Clarendon Press, pp. 97-162.
Xiong D & Flexas J. 2020. From one side to two sides: the effects of stomatal distribution on photosynthesis. New Phytologist 228(6): 1754-1766. https://doi.org/10.1111/nph.16801
Zini ADS, Martins S, Toderke ML & Temponi LG. 2016. Anatomia foliar de Rubiaceae ocorrentes em fragmento florestal urbano de Mata Atlântica, PR, Brasil. Hoehnea 43(2): 173-182. https://doi.org/10.1590/2236-8906-59/2015
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