THE EFFECT OF CHITOSAN IN THE DEVELOPMENT AND MORPHOLOGY OF ALTERNARIA ALTERNATA (FR.) KEISSL

Authors

  • Delfino Sánchez-Domínguez
  • Silvia Bautista-Baños
  • Patricia Castillo Ocampo
Keywords: chitosan molecular weight, esporulation, germination, micelium growth, scanning electronic microscopy

Abstract

The potential of low and medium molecular weight chitosan (LMWC, MMWC) at five concentrations (0.5, 1.0, 1.5, 2.0 and 2.5% w/v) and high molecular weight chitosan (HMWC) at four concentrations (0.5, 1.0, 1.5 and 2.0 w/v) to inhibit the fungus Alternaria alternata was evaluated. MMWC at 2.5% w/v inhibited mycelial growth of A. alternata up to 50.6%, whilst 0.5, 1.0 and 1.5% doses at the three molecular weight promoted mycelial growth. Sporulation was affected at 2.5% concentration by LMWC and MMWC. Percentage of spore germination was not affected by concentration but it was affected by molecular weight of chitosan. An inverse relationship was observed between germination and molecular weight. Mycelial dry weight was inhibited by all concentrations and type of chitosan evaluated. Observations at the scanning electron microscopy showed diverse alterations in hyphaes such as swelling, depression, and distortion by effect of chitosan.

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Author Biographies

Delfino Sánchez-Domínguez

Dirección General de Educación Tecnológica Agropecuaria Centro de Bachillerato Tecnológico Agropecuario México

Silvia Bautista-Baños

Instituto Politécnico Nacional Centro de Desarrollo de Productos Bióticos México

Patricia Castillo Ocampo

Universidad Autónoma Metropolitana- Iztapalapa Laboratorio de Microscopía Electrónica México
How to Cite
Sánchez-Domínguez, D., Bautista-Baños, S., & Castillo Ocampo, P. (2007). THE EFFECT OF CHITOSAN IN THE DEVELOPMENT AND MORPHOLOGY OF ALTERNARIA ALTERNATA (FR.) KEISSL. Anales de Biología, (29), 23–32. Retrieved from https://revistas.um.es/analesbio/article/view/40741
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