Somatic embryogenesis in Allium sativum L. (cv. Yamuna Safed 3): Improving embryo maturation and germination with PGRs and carbohydrates

Authors

  • Sekh Abdul Nasim
  • Abdul Mujib
  • Rashmi Kapoor
  • Samar Fatima
  • Junaid Aslam & Mahmooduzzafar
Keywords: Allium sativum L., Callus, Carbon Sources, Plant Growth Regulators, Somatic Embryos

Abstract

Callus induction was obtained from basal part of the garlic clove on Murashige and Skoog (MS) medium on which somatic embryos were formed. In Allium sativum L. cv. Yamuna Safed 3, somatic embryogeny has been categorized into three distinct stages: initiation and proliferation of embryo; maturation; and germination of embryo. The initiation of globular-stage embryo differentiation was observed on various BAP and 2,4-D concentrations but at 1.0 mg/l BAP and 0.25 mg/l 2,4-D maximum proliferation of embryos was noted. Best somatic embryo maturation was achieved on 0.5 mg/l GA 3 amended medium; ABA at the same level was less effective. Embryo growth was maximum on 3% maltose while plantlet conversion was highest (65.15%) on 3% fructose added medium. Scanning electron microscopy (SEM) has been presented to demonstrate the apparent morphology and structural details of embryo. Plantlets were successfully raised from in vitro developed embryos.

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How to Cite
Abdul Nasim, S., Mujib, A., Kapoor, R., Fatima, S., & Aslam & Mahmooduzzafar, J. (2010). Somatic embryogenesis in Allium sativum L. (cv. Yamuna Safed 3): Improving embryo maturation and germination with PGRs and carbohydrates. Anales de Biología, (32), 1–9. Retrieved from https://revistas.um.es/analesbio/article/view/264151
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