Estimation of nuclear DNA content in Algerian populations of <em>Atriplex halimus</em> and <em>Atriplex canescens</em> by flow cytometry

Authors

  • Miloud Aouissat
  • Moulay Belkhoja
  • Kheiria Hcini
  • David J. Walker
  • Enrique Correal
Keywords: <em>Atriplex halimus</em>, <em>Atriplex canescens</em>, flow cytometry, nuclear DNA content

Abstract

Atriplex halimus is a perennial native shrub of the Mediterranean Basin with an excellent tolerance to drought and salinity, also, Atriplex canescens, four-wing saltbush, is adapted to desert climates. The aim of this work was to estimate nuclear DNA content in Algerian populations of A. halimus and A. canescens by flow cytometry, an efficient method to estimate ploidy levels and to de- termine nuclear DNA content in plants. Flow cytomertry is a technique which permits rapid estimation of nuclear DNA content. With respect to nuclear DNA, the 2C DNA content of population Cala Tarida (Spain) was estimated to be 2.46pg. There was no significant difference among the populations of A. halimus, whose 2C DNA content ranged from 4.91 to 5.04pg. However, the 2C DNA content of A. canescens ranged from 3.14 to 3.27.

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Author Biographies

Miloud Aouissat

Centre Universitaire de SaidaInstitut des Sciences de la Nature et de la VieDépartement de Biologie20100 Ain- El- HadjarSaidaAlgerie

Moulay Belkhoja

Université d’Oran Es-SeniaLaboratoire de Physiologie Végétale

Kheiria Hcini

Faculté des Sciences de TunisDépartement des Sciences Biologiques2092 El-Manar II TunisTunisia

David J. Walker

Departamento de Recursos Naturales y Desarrollo RuralInstituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA)La Alberca30150 MurciaSpain

Enrique Correal

Departamento de Recursos Naturales y Desarrollo RuralInstituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA)La Alberca30150 MurciaSpain
How to Cite
Aouissat, M., Belkhoja, M., Hcini, K., Walker, D. J., & Correal, E. (2009). Estimation of nuclear DNA content in Algerian populations of <em>Atriplex halimus</em> and <em>Atriplex canescens</em> by flow cytometry. Anales De Biología, (31), 15–18. Retrieved from https://revistas.um.es/analesbio/article/view/110531
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