Aislamiento y caracterización de FS01, un bacteriófago lítico que infecta a Salmonella typhimurium
Resumen
La contaminación por Salmonella representa un problema sanitario y económico. Este estudio aisló y caracterizó un bacteriófago lítico, FS01, activo contra S. typhimurium. FS01 mostró una MOI de 1, tasa de adsorción de 1,9x10-9 mL/min, y una curva de crecimiento con eclipse de 10 minutos, latencia de 15 minutos y tamaño de explosión de 10 viriones por célula. Posee cápside icosaédrica (64,4 nm) y cola flexible (182,2 nm), lo que sugiere pertenecer a la familia Siphoviridae, con estrecho rango de hospedero; es estable térmicamente entre 40-60 ºC por 30 minutos, viable entre pH 6–9, sensible a cloroformo y luz UV. FS01 inhibió el crecimiento bacteriano desde las 3 horas. FS01 presenta propiedades de un agente biocontrolador prometedor para descontaminar alimentos y superficies.
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