Synthesis of calcitonin gene-related peptide (CGRP) by rat arterial endothelial cells

  • Yoshiaki Doi University of Occupational and Environmental Health
  • H. Kudo University of Occupational and Environmental Health
  • T. Nishino University of Occupational and Environmental Health
  • K. Kayashima University of Occupational and Environmental Health
  • H. Kiyonaga University of Occupational and Environmental Health
  • T. Nagata University of Occupational and Environmental Health
  • S. Nara University of Occupational and Environmental Health
  • M. Morita University of Occupational and Environmental Health
  • S. Fujimoto University of Occupational and Environmental Health
Keywords: calcitonin gene-related peptide, In situ, hybridization, immunocytochemistry, immunoelectron microscopy, Weibel-Palade bodies

Abstract

We investigated the protein and mRNA expression of calcitonin gene-related peptide (CGRP) in endothelial cells of the rat thoracic aorta and femoral artery. Light microscopic immunocytochemistry revealed that immunoreactivity for CGRP was preferentially located in the endothelium of both vessels. Immunoelectron microscopy showed that CGRPimmunoreactive gold particles were preferentially localized on cisterns of the rough endoplasmic reticulum and on the Weibel-Palade (WP) bodies in the endothelial cells. Prepro CGRP mRNA signals were also detected on the endothelium. Our results are the first to demonstrate that endothelial cells of both elastic and large muscular arteries synthesize CGRP and store it, in part, in WP bodies, implying that CGRP may act as an endothelium-derived relaxing factor in these vessels.

Author Biographies

Yoshiaki Doi, University of Occupational and Environmental Health
Department of Anatomy
H. Kudo, University of Occupational and Environmental Health
Department of Anatomy
T. Nishino, University of Occupational and Environmental Health
Department of Anatomy
K. Kayashima, University of Occupational and Environmental Health
Department of Anatomy
H. Kiyonaga, University of Occupational and Environmental Health
Department of Anatomy
T. Nagata, University of Occupational and Environmental Health
Department of Anatomy
S. Nara, University of Occupational and Environmental Health
Department of Anatomy
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Articles