Efectos citotóxicos del paracetamol (acetaminofeno) en células meristemáticas y germinación de semillas de Allium cepa L.
Agencias de apoyo
- Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
Resumen
El desecho de medicamentos en la basura doméstica puede ser muy peligrosa para el medio ambiente. El presente trabajo buscó evaluar parámetros de toxicidad del contaminante emergente paracetamol. En los ensayos se utilizaron tres dosis de paracetamol (0,4 mg L-1, 0,5 mg L-1 y 0,6 mg L-1). Los resultados mostraron que la exposición aguda al paracetamol induce varios daños clastogénicos y aneugénicos en el test de A. cepa. No se encontró ningún efecto en la germinación. Aunque el paracetamol es un fármaco importante, también puede representar un riesgo significativo para la producción agrícola y la preservación del medio ambiente. Los resultados del presente trabajo corroboran datos anteriores y refuerzan que la exposición a contaminantes farmacéuticos puede representar un problema significativo para la producción de alimentos, el equilibrio ambiental y la preservación de especies.
Descargas
Citas
Anacleto LR, Roberto MM & Marin-Morales MA. 2017. Toxicological effects of the waste of the sugarcane industry, used as agricultural fertilizer, on the test system Allium cepa. Chemosphere 173: 31-42. https://doi.org/10.1016/j.chemosphere.2017.01.033
Ancia JP & Romão NF. 2016. Análise da atividade citotóxica e mutagênica do extrato aquoso das partes aéreas de Uncaria tomentosa em teste de Allium cepa. South American Journal of Basic Education, Technical and Technological 3: 16-26.
Ayres M, Ayres Junior M, Ayres DL & Santos AAS. 2007. BIOESTAT 5.3 – Aplicações estatísticas nas áreas das ciências biomédicas. Belém: Instituto Mamirauá. 364p.
Bagatini MD, Silva ACF & Tedesco SB. 2007. The use of Allium cepa test as a bioindicator of genotoxicity of medicinal plants infusions. Revista Brasileira de Farmacognosia 17(3): 444-447. https://doi.org/10.1590/S0102-695X2007000300019
Bergman K, Müller L & Teigen SW. 1996. The genotoxicity and carcinogenicity of paracetamol: a regulatory (re)view. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 349(2): 263-288. https://doi.org/10.1016/0027-5107(95)00185-9
Bianchi J, Espindola ELG & Marin-Morales MA. 2011. Genotoxicity and mutagenicity of water samples from the Monjolinho River (Brazil) after receiving untreated effluents. Ecotoxicology and Environmental Safety 74: 826-833. https://doi.org/10.1016/j.ecoen v.2010.11.006
Bernardes PM, Andrade-Vieira LF, Aragão FB, Ferreira A & Da Silva Ferreira MF. 2015. Toxicity of Difenoconazole and Tebuconazole in Allium cepa. Water, Air & Soil Pollution 226(7): 1-11. https://doi.org/10.1007/s11270-015-2462-y
Brasil. 2009. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Brasília, 365 p.
Bridges EM & Oldeman LR. 1999. Global Assessment of Human Induced Soil Degradation. Arid Soil Research and Rehabilitation 13(4): 319-325. https://doi.org/10.1080/089030699263212
Camargo WP & Camargo, FP. 2017. A quick review of the production and commercialization of the main vegetables in Brazil and the world from 1970 to 2015. Horticultura Brasileira 35: 160-166. https://doi.org/10.1590/S0102-053620170202
Cortés-Eslava J, Gómez-Arroyo S, Risueño MC & Testillano PS. 2018. The effects of organophosphorus insecticides and heavy metals on DNA damage and programmed cell death in two plant models. Environmental Pollution 240: 77-86. https://doi.org/10.1016/j.envpol.2018.04.119
Chrois KM, Larsen S, Pedersen JS, Rygg MO, Boilsen AEB, Bendtsen F & Dela F. 2020. Acetaminophen toxicity induces mitochondrial complex I inhibition in human liver tissue. Basic & Clinical Pharmacology & Toxicology 126(1): 86-91. https://doi.org/10.1111/bcpt.13304
Couto CF, Lange LC & Amaral MCS. 2019. Occurrence, fate and removal of pharmaceutically active compounds (PhACs) in water and wastewater treatment plants - A review. Journal of Water Process Engineering 32: 100927. https://doi.org/10.1016/j.jwpe.2019.100927
Cuchiara CC, Borges CS & Bobrowski VL. 2012. Sensibilidade de sementes de hortaliças na avaliação da qualidade da água em bioensaios. Biotemas 25 (3): 19-27. https://doi.org/10.5007/2175-7925.2012v25n3p19
Da Silva Júnior JG, Santos NSDS, Araújo HDA, Sá BDC, Do Nascimento Júnior JAA, Maciel NGP, Araújo SRR, Araújo VFS, Callou MAM & Monte TVS. 2019. Hepatotoxicidade induzida pelo paracetamol e a utilização do nomograma de Rumack-Matthew para avaliar a terapêutica com N-Acetilcisteína. Revista Uningá 56(4): 65-84. https://doi.org/10.46311/2318-0579.56.eUJ2087
Fisher ES & Curry SC. 2019. Evaluation and treatment of acetaminophen toxicity. Advances in Pharmacology 85: 263-272. https://doi.org/10.1016/bs.apha.2018.12.004
Fiskesjö, G. 1958. The Allium-test as a standard in environmental monitoring, Hereditas, 102: 99–112. https://doi.org/10.1111/j.1601-5223.1985.tb00471.x
Garrido RM. 2012. Extração e Quantificação de Paracetamol em Fármacos. São Paulo: Instituto Municipal de Ensino Superior de Assis. 57p. Avaiable at https://cepein.femanet.com.br/BDigital/arqTccs/0911290928.pdf
Grover IS & Kaur S. 1999. Genotoxicity of wastewater samples from sewage and industrial effluent detected by the Allium root anaphase aberration and micronucleus assays. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 426(2): 183-188. https://doi.org/10.1016/S0027-5107(99)00065-2
Hongslo JK, Bjørge C, Schwarze PE, Brøgger A, Mann G, Thelander L, & Holme JA. 1990. Paracetamol inhibits replicative DNA synthesis and induces sister chromatid exchange and chromosomal aberrations by inhibition of ribonucleotide reductase. Mutagenesis 5(5): 475-480. https://doi.org/10.1093/mutage/5.5.475
Krupina K, Goginashvili A & Cleveland DW. 2021. Causes and consequences of micronuclei. Current Opinion in Cell Biology 70: 91–99. https://doi.org/10.1016/j.ceb.2021.01.004
Lessa LR & Cariello FMR. 2017. Adsorção do paracetamol em carvão ativado: regressão da citotoxicidade e mutagênicidade no sistema Allium cepa HÓRUS 12(1): 44-54.
Lessa LR, Da Silva MCC & Cariello FMR. 2017. Fundamentos e aplicações do Allium cepa como bioindicador de mutagenicidade e citotoxicidade de plantas medicinais. Revista Intertox de Toxicologia, Risco Ambiental e Sociedade 10(3): 39-48. https://doi.org/10.22280/revintervol10ed3.294
Lima DRS, Tonucci MC, Libânio M & Aquino SF. 2017. Fármacos e desreguladores endócrinos em águas brasileiras: ocorrência e técnicas de remoção. Engenharia Sanitária e Ambiental 22(6): 1043-1054. https://doi.org/10.1590/S1413-41522017165207
Lima MGF, Rocha LC, Silveira GL, Alvarenga IFS, Andrade-Vieria LF. 2019. Nucleolar alterations are reliable parameters to determine the cytogenotoxicity of environmental pollutants. Ecotoxicology and Environmental Safety 15(174): 630-636. https://doi.org/10.1016/j.ecoenv.2019.03.033
Liu D & Kottke I. 2003. Subcellular localization of chromium and nickel in root cells of Allium cepa by EELS and ESI. Cell Biology and Toxicology 19: 299–311. https://doi.org/10.1023/B:CBTO.0000004984.87619.15
Liu B & Lee YJ. 2022. Spindle Assembly and Mitosis in Plants. Annual Review of Plant Biology 20 (73): 227-254. https://doi.org/10.1146/annurev-arplant-070721-084258
Livanos P, Apostolakos P & Galatis B. 2012. Plant cell division. Plant Signaling & Behavior 7(7): 771-778. https://doi.org/10.4161/psb.20530
Machado AT. 2013. Avaliação do potencial mutagênico do efluente do Terminal Petroquímico Almirante Soares Dutra (Osório-RS-Brasil) através do sistema teste de Allium cepa. Osório (BR): Universidade Estadual do Rio Grande do Sul. Dissertation.
Monteavaro ML. 2014. Comercialização da cultura de cebola no município de Tavares/RS. Curitiba (BR): Universidade Federal do Paraná. Dissertation.
Nascimento RF, Carvalho Filho JAA., Napoleão DC, Ribeiro BG, Silva Pereira Cabral JJ. & Paiva ALR. 2023. Presence of non-steroidal anti-inflammatories in Brazilian semiarid waters. Water, Air, & Soil Pollution, 234(4): 225. https://doi.org/10.1007/s11270-023-06239-2
Nunes B. 2020. Ecotoxicological Effects of the Drug Paracetamol: A Critical Review of Past Ecotoxicity Assessments and Future Perspectives. In: (Gómez-Oliván, L.M. eds) Non-Steroidal Anti-Inflammatory Drugs in Water. The Handbook of Environmental Chemistry, 96.Springer Nature
OECD. 2006. Test No. 208: Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test, OECD Guidelines for the Testing of Chemicals, Section 2, OECD Publishing, Paris, https://doi.org/10.1787/9789264070066-en.
Pacheco-Álvarez M, Benítez RP, Rodríguez-Narváez OM, Brillas, E & Peralta-Hernández JM. 2022. A critical review on paracetamol removal from different aqueous matrices by Fenton and Fenton-based processes, and their combined methods. Chemosphere 303: 134883. https://doi.org/10.1016/j.chemosphere.2022.134883
Peralta-Hernández JM & Brillas E. 2023. A critical review over the removal of paracetamol (acetaminophen) from synthetic waters and real wastewaters by direct, hybrid catalytic, and sequential ozonation processes. Chemosphere 313: 137411. https://doi.org/10.1016/j.chemosphere.2022.137411
Pereira BVR. 2018. Acute and chronic effects of the pharmaceuticals paracetamol and propranolol in different biomarkers of a neotropical fish species. Sorocaba (BR): Universidade Federal de São Carlos. Dissertation.
Pereira MAM. 2018. Mecanismo de Hepatotoxicidade do Paracetamol. Porto (PT): Universidade do Porto. Dissertation
Peters NE & Meybck M. 2000. Water Quality Degradation Effects on Freshwater Availability: Impacts of Human Activities. Water International. 25(2):185-193. https://doi.org/10.1080/02508060008686817
Pinheiro GS, Angelotti F, Santana CVS, Dantas BF & Costa ND. 2014. Efeito da temperatura sobre a germinação de sementes de cebola. Scientia Plena 10(11): 1-6.
Pinho EAS, Ferreira LFR, Américo-PinheirO JHP & Torres NH. 2017. Tratamento de esgoto sanitário contendo micropoluentes no Brasil: revisão. Bioenergia em Revista: Diálogos 7(2): 46-63.
Prajitha V & Thoppil JE. 2016. Genotoxic and antigenotoxic potential of the aqueous leaf extracts of Amaranthus spinosus Linn. using Allium cepa assay. South African Journal of Botany 102: 18-25. https://doi.org/10.1016/j.sajb.2015.06.018
Priyan V, Shahnaz T, Suganya E, Sivaprakasam S & Narayanasamy S. 2021. Ecotoxicological assessment of micropollutant Diclofenac biosorption on magnetic sawdust: Phyto, Microbial and Fish toxicity studies. Journal of Hazardous Materials 403: 123532. https://doi.org/10.1016/j.jhazmat.2020.123532
Remião FO. 2020. Paracetamol e a COVID-19. Revista de Ciência Elementar 8(2): 023. http://doi.org/10.24927/rce2020.023
Rodrigues DD, Santos BK, Silva RS, Silva RR, Santana DL & Santos WMS. 2016. Padronização da Técnica Allium cepa para pesquisa de danos mutagênicos em infusão de plantas na cidade de Guaraí. Revista Eletrônica de Farmácia 6(1): 90-99.
Rogowska J, Cieszynska-Semenowicz M, Ratajczyk W & Wolska L. 2020. Micropollutants in treated wastewater. Ambio 49: 487–503. https://doi.org/10.1007/s13280-019-01219-5
Rosculete CA, Bonciu E, Rosculete E & Olaru LA. 2019. Determination of the environmental pollution potential of some herbicides by the assessment of cytotoxic and genotoxic effects on Allium cepa. International Journal of Environmental Research and Public Health 16(1): 75. https://doi.org/10.3390/ijerph16010075
Rossetti CR, Da Silva Almeida A, Medeiros LB, Cortez AM, Salbego MS, Aguiar RN & De Tunes LVM. 2019. Condições de substrato e temperatura para condução do teste de germinação de sementes de Allium cepa L. e Allium fistulosum L. Brazilian Journal of Development 5(9): 17042-17048. https://doi.org/10.34117/bjdv5n9-226
Sabeen M, Mahmood Q, Ahmad Bhatti Z, Faridullah Irshad M, Bilal M, ... Shahid N. 2020. Allium cepa assay based comparative study of selected vegetables and the chromosomal aberrations due to heavy metal accumulation. Saudi Journal of Biological Sciences 27(5): 1368-1374. https://doi.org/10.1016/j.sjbs.2019.12. 011
Sousa AP & Nunes B. 2021. Dangerous connections: biochemical and behavioral traits in Daphnia magna and Daphnia longispina exposed to ecologically relevant amounts of paracetamol. Environmental Science and Pollution Research volume 28: 38792–38808. https://doi.org/10.1007/s11356-021-13200-5
Sousa B, Lopes J, Leal A, Martins M, Soares C, Valente Im, Rodrigues Ja, Fidalgo F & Teixeira J. 2020. Response of Solanum lycopersicum L. to diclofenac – Impacts on the plant’s antioxidant mechanisms. Environmental Pollution 258: 113762. https://doi.org/10.1016/j.envpol.2019.113762
Sparemberguer RFL & Da Silva DA. 2005. A Relação Homem, Meio Ambiente, Desenvolvimento e o Papel do Direito Ambiental. Veredas do Direito. 2 (4): 81-99. https://doi.org/10.18623/rvd.v2i4.103
Sutan NA, Popescu A, Mihaescu C, Soare LC & Marinescu MV. 2014. Evaluation of cytotoxic and genotoxic potential of the fungicide Ridomil in Allium cepa L. Analele Ştiinţifice ale Universităţii Al. I. Cuza” Iaşi s. II a. Biologie vegetală. 60(1): 5-12.
Sturbelle RT, Pinho DS, Restani RG, Oliveira GR, Garcias GL & Roth MGM. 2010. Avaliação da atividade mutagênica e antimutagênica da Aloe vera em teste de Allium cepa e teste de micronúcleo em linfócitos humanos binucleados. Revista Brasileira de Farmacognosia 20(3): 409-415. https://doi.org/10.1590/S0102-695X2010000300019
Wadhah Hassan AE, Ali Gamal AK, Khalid Mohammed N & Qais Yusuf MA. 2017. Occurrence of paracetamol in aquatic environments and transformation by microorganisms: a review. Chronicles of Pharmaceutical Science 1: 341-355.
Zhu JK. 2016. Abiotic stress signaling and responses in plants. Cell, 167(2): 313-324. https://doi.org/10.1016/j.cell.2016.08.029
Derechos de autor 2025 Anales de Biología
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
Las obras que se publican en esta revista están sujetas a los siguientes términos:
1. El Servicio de Publicaciones de la Universidad de Murcia (la editorial) conserva los derechos patrimoniales (copyright) de las obras publicadas, y favorece y permite la reutilización de las mismas bajo la licencia de uso indicada en el punto 2.
2. Las obras se publican en la edición electrónica de la revista bajo una licencia Creative Commons Reconocimiento-NoComercial-SinObraDerivada 3.0 España (texto legal). Se pueden copiar, usar, difundir, transmitir y exponer públicamente, siempre que: i) se cite la autoría y la fuente original de su publicación (revista, editorial y URL de la obra); ii) no se usen para fines comerciales; iii) se mencione la existencia y especificaciones de esta licencia de uso.
3. Condiciones de auto-archivo. Se permite y se anima a los autores a difundir electrónicamente las versiones pre-print (versión antes de ser evaluada) y/o post-print (versión evaluada y aceptada para su publicación) de sus obras antes de su publicación, ya que favorece su circulación y difusión más temprana y con ello un posible aumento en su citación y alcance entre la comunidad académica. Color RoMEO: verde.
Biological Abstracts, BIOSIS Previews, Zoological Record
